Anti-phospho-TDP-43 [pSer409] antibody produced in rabbit
SIGMA/SAB4200223 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-phospho-ALS10; Anti-phospho-TAR DNA binding protein 43; Anti-phospho-TARDBP; Anti-phospho-TARDP43
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluoresence. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Anti-phospho-TDP-43 (pSer410) antibody produced in Rabbit (Cat. No. SAB4200224) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC Conjugate antibody, and 2 μg/mL Anti-phospho-TDP-43 [pSer409] antibody produced in Rabbit (Cat. No. SAB4200223) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody. Results Two Anti phospho-TDP-43 antibodies, SAB4200224 (A) and SAB4200223 (B) target different regions of phospho-TDP-43 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence Cytoplasmic and nuclear localization of phospho-TDP-43 [pSer409] in HeLa cells. Fixed cells were stained with 1 μg/mL Anti-phospho-TDP-43 [pSer409] (Cat. No. SAB4200223) followed by Goat anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Cells extracts were separated on SDS-PAGE, blotted with 1 μg/mL Anti-phospho-TDP-43 [pSer409] (Cat. No. SAB4200223), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. A431 2. HeLa 3. K562 4. HepG2 5. HepG2. Antibody + non-phospho-TDP-43 peptide 6. HepG2. Antibody + immunizing phospho-TDP-43 [pSer409] peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~43 kDa |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer409) |
| technique(s) | indirect immunofluorescence: 1-2 μg/mL using HeLa cells |
| western blot: 1-2 μg/mL using HepG2 and A431 cell lysates | |
| UniProt accession no. | Q13148   |
| Application: | Anti-phospho-TDP-43 [pSer409] antibody produced in rabbit has been used in: • enzyme linked immuno sorbent assay (ELISA) • immunohistochemistry • immunoblotting • immunofluorescence |
| Biochem/physiol Actions: | Transactive response DNA-binding protein 43 (TDP-43) binds to single stranded RNA. It regulates transcription regulation in human immunodeficiency virus (HIV). TDP-43 is the major ubiquinated component of cytoplasmic inclusions in frontotemporal lobe degeneration subtype (FTLD-U) and amyotrophic lateral sclerosis (ALS). Pathological TDP-43 forms abnormal inclusions in neuronal perikarya and neurites. Mutations in TDP-43 is associated with ALS. Abnormal phosphorylation of TDP-43 at Ser409/410 is observed in FTLD-U and ALS, leading to apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Transactive response DNA-binding protein 43 (TDP-43) belongs to the family of heterogeneous nuclear ribonucleoproteins (hnRNPs). It has two RNA-recognition motifs and a glycine-rich C-terminal sequence. TDP-43 is expressed in heart, lung, liver and brain, which is localized to the nucleus.. |
| Immunogen: | synthetic peptide containing phosphorylated Ser409 of human TDP-43 conjugated to KLH. The corresponding sequence is identical in mouse TDP-43 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |