Anti-phospho-TDP-43 (pSer410) antibody produced in rabbit
SIGMA/SAB4200224 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-phospho-ALS10; Anti-phospho-TAR DNA binding protein 43; Anti-phospho-TARDBP; Anti-phospho-TARDP43
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluoresence. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Anti-phospho-TDP-43 (pSer410) antibody produced in Rabbit (Cat. No. SAB4200224) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC Conjugate antibody, and 2 μg/mL Anti-phospho-TDP-43 [pSer409] antibody produced in Rabbit (Cat. No. SAB4200223) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody. Results Two Anti phospho-TDP-43 antibodies, SAB4200224 (A) and SAB4200223 (B) target different regions of phospho-TDP-43 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunoblotting Cells extracts were separated on SDS-PAGE, blotted with 1 μg/mL Anti-phospho-TDP-43 [pSer410] (Cat. No. SAB4200224), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa 2. K562 3. A431 4. HEK293 5. NIH3T3 6. HepG2 7. HepG2. Antibody + non-phospho-TDP-43 peptide 8. HepG2. Antibody + immunizing phospho-TDP-43 [pSer410] peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~43 kDa |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer410) |
| technique(s) | western blot: 1-2 μg/mL using HepG2 and NIH3T3 cell lysates |
| UniProt accession no. | Q13148   |
| Application: | Anti-phospho-TDP-43 (pSer410) antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | Pathological TDP-43 forms abnormal inclusions in neuronal perikarya and neurites. Several pathogenic TDP-43 mutations have been identified in familial amyotrophic lateral sclerosis (ALS). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | TDP-43 (TAR DNA binding protein) belongs to the family of heterogeneous nuclear ribonucleoproteins (hnRNPs), that binds single stranded RNA. TDP-43 has been implicated in the transcription regulation of HIV. TDP-43 is the major ubiquinated component of cytoplasmic inclusions in frontotemporal lobe degeneration subtype FTLD-U and amyotrophic lateral sclerosis (ALS). TDP-43 is predominantly localized to the nucleus. |
| Immunogen: | synthetic peptide containing phosphorylated Ser410 of human TDP-43, conjugated to KLH. The corresponding sequence is identical in mouse TDP-43. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |