Anti-DCAL-1 antibody, Mouse monoclonal
SIGMA/SAB4200231 - clone UW50, purified from hybridoma cell culture
Synonym: Anti-C-type lectin-like 1; Anti-CLECL1
Product Type: Chemical
Flow Cytometry DCAL-1 expression on Raji cells: 1×106 Raji cells were labelled with 5 μg/mL Monoclonal Anti-DCAL-1 Clone: UW50 (Cat. No. SAB4200231) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Shaded histogram: antibody-specific staining Gray histogram: negative control without first antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | UW50, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgM |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 0.25-0.50 μg/test using 1x106 human Raji cells |
| UniProt accession no. | Q8IZS7   |
| Application: | Monoclonal Anti-DCAL-1 antibody produced in mouse may be used in flow cytometry. |
| Biochem/physiol Actions: | Dendritic cell-associated lectin-1 (DCAL-1) functions as a T-cell co-stimulatory molecule and increases interleukin-4 secretion. The encoded protein is also implicated in the regulation of the immune response. |
| Biochem/physiol Actions: | Monoclonal Anti-DCAL-1 recognizes human DCAL-1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Dendritic cell-associated lectin-1 (DCAL-1), also known as C-type lectin-like-1 (CLECL1), is a novel C-type lectin-like molecule, encoded by the gene mapped to human chromosome 12p13.31. The encoded protein is exclusively expressed in human hematopoietic cells, in particular at high levels in dendritic cells (DCs) and B cells. |
| General description: | Monoclonal Anti-DCAL-1 (mouse IgM isotype) is derived from the hybridoma UW50 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a human DCAL-1 fusion protein |
| Immunogen: | human DCAL-1 fusion protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |