Anti-GAD65 antibody , Mouse monoclonal
SIGMA/SAB4200232 - clone N-GAD65, purified from hybridoma cell culture
Synonym: Anti-GAD2; Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Anti-glutamate decarboxylase 65 kDa isoform
MDL Number: MFCD03455043
Product Type: Chemical
Western Blotting Whole extracts of Rat brain were separated on SDS-PAGE and probed with Monoclonal Anti-GAD65 antibody produced in Mouse, Clone: N-GAD65 (Cat. No. SAB4200232). The antibody was developed using 1:20,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. 0.25 μg/mL antibody 2. 0.5 μg/mL antibody 3. 1 μg/mL antibody 4. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | N-GAD65, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~65 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, monkey, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 0.25-0.5 μg/mL using whole extracts of rat brain | |
| UniProt accession no. | Q05329  |
| Application: | Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA. |
| Biochem/physiol Actions: | Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |