Anti-TGN46 antibody, Mouse monoclonal
SIGMA/SAB4200235 - clone TGN46-52, purified from hybridoma cell culture
Synonym: Anti-Trans-Golgi network protein, 46-kD
MDL Number: MFCD09265378
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing human TGN46 was separated on SDS-PAGE and probed with Monoclonal Anti-TGN46 Clone: TGN46-52 (Cat. No. SAB4200235). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + TGN46 immunizing peptide 3. 1 μg/mL antibody 4. Negative control: without first antibody
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-TGN46 Clone: TGN46-52 (Cat. No. SAB4200235). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HEK-293T cells overexpressing human TGN46 3. HeLa
Immunoprecipitation 10 μg/mL of Monoclonal Anti-TGN46 Clone: TGN46-52 (Cat. No.SAB4200235) were used to immunoprecipitate TGN46 from whole cell extracts. Detection antibody: Rabbit Anti-TGN46 antibody (Cat. No. T7576). Lanes 1. HEK-293T cells overexpressing human TGN46 2. HeLa
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TGN46-52, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 80-100 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human TGN46 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper)  |
| Application: | Monoclonal Anti-TGN46 antibody produced in mouse has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Trans-Golgi network protein, 46-kDa (TGN46) is involved in regulating membrane traffic to and from the TGN. It cycles constitutively between the TGN and the plasma membrane, returning via endosomes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-TGN46 (mouse IgG1 isotype) is derived from the hybridoma TGN46-52 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human TGN46 conjugated to KLH. Trans-Golgi network protein 46-kDa (TGN46), It is a heavily glycosylated protein, containing a signal peptide, a lumenal domain, a membrane-spanning domain and a cytoplasmic domain. The membrane spanning region and the cytoplasmic tail contain the retention and retrieval signals for localization in the TGN. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |