Anti-Glutathione-S-transferase (GST) antibody, Mouse monoclonal
SIGMA/SAB4200237 - clone 2H3-D10, purified from hybridoma cell culture
Synonym: Mouse Anti-GST Tag
MDL Number: MFCD02253002
Product Type: Chemical
Immunoblotting GST recombinant protein was separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-GST Clone: 2H3-D10 (Cat. No. SAB4200237). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.05 μg GST 2. 0.025 μg GST 3. 0.0125 μg GST 4. 0.006 μg GST
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2H3-D10, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.2-0.4 μg/mL using detection limit for GST-fustion protein is ∼ 10ng/lane |
| Application: | Anti-Glutathione-S-transf |
| Biochem/physiol Actions: | Glutathione-S-Transferase (GST) is an enzyme, which catalyzes the reaction of glutathione with a wide range of electrophilic substrates and therefore, plays a role in the detoxification of potential alkylating agents.(5) GST protects the cell against oxidative stress and several toxic molecules. It facilitates the synthesis and modification of leukotrienes and prostaglandins. GST from Schistosoma japonicum, has been cloned in several expression vectors as a 27.5 kDa molecule and used as a tag for expressing proteins of interest. These proteins are thus expressed as GST-fusion proteins. Antibodies to GST are useful in identifying the successful expression of these fusion proteins. |
| Biochem/physiol Actions: | Monoclonal Anti- Glutathione-S-transferase (GST) recognizes GST from Schistosoma japonicum in GSTfusion proteins. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Glutathione-S-transf |
| Immunogen: | hybridoma 2H3-D10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a GST-fusion protein |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | Store at −20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |