Anti-PTPN2 antibody produced in rabbit
SIGMA/SAB4200249 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-PTN2; Anti-PTPT; Anti-TC-PTP; Anti-TCELLPTP; Anti-TCPTP
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-PTPN2 antibody produced in Rabbit (Cat. No. SAB4200249) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing PTPN2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.
Immunoblotting Tissue extracts and cell lysates were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-PTPN2 (Cat. No. SAB4200249), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat kidney (S1 fraction) 2. Mouse kidney (S1 fraction) 3. Jurkat 4. HEK293-T overexpressing human PTPN2 5. HEK293-T non-transfected 6. Rat kidney (S1 fraction): Antibody + immunizing PTPN2 peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~48 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1.5-3.0 μg/mL using Jurkat cell extracts, rat kidney extracts (S1 fraction) or mouse kidney extracts (S1 fraction). |
| UniProt accession no. | P17706  |
| Application: | Anti-PTPN2 antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunostaining |
| Biochem/physiol Actions: | Protein tyrosine phosphatase non-receptor type 2 (PTPN2) is a tyrosine phosphatase that negatively regulates tyrosine kinase and JAK/STAT signaling pathways. PTPN2 inhibits oncogenic JAK1 and thereby functions as a tumor suppressor in T-cell malignancies. Genetic alterations in PTPN2 have been associated with the risk of Crohn′s disease and ulcerative colitis . |
| Biochem/physiol Actions: | PTPN2 acts on several cytoplasmic substrates including epidermal growth factor receptor (EGFR), insulin receptor (InsR), Shc, janus kinases (JAKs), and the nuclear substrate signal transducer and activator of transcription 1 (STAT1). PTPN2 has been associated with type 1 diabetes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Protein tyrosine phosphatase non-receptor type 2 (PTPN2), also known as T cell protein tyrosine phosphatase (TCPTP), is an intracellular nonreceptor tyrosine-specific phosphatase that is expressed ubiquitously at various stages of mammalian development. PTPN2 exists as two alternatively spliced variants, PTPN2 isoform 1 (p48TC, 48 kDa) that is targeted to the endoplasmic reticulum by a hydrophobic C-terminal region and a shorter PTPN2 isoform 2 (p45TC, 45 kDa) that is targeted to the nucleus. |
| Immunogen: | synthetic peptide corresponding to a sequence located near the C-terminus of human PTPN2, conjugated to KLH. The corresponding sequence is identical in human PTPN2 isoform 2 and highly conserved (single amino acid substitution) in mouse PTPN2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |