Anti-AKT1S1 antibody, Mouse monoclonal
SIGMA/SAB4200256 - clone AKT1S1-3, purified from hybridoma cell culture
Synonym: Monoclonal Anti-AKT1 substrate 1 (proline-rich); Monoclonal Anti-Lobe; Monoclonal Anti-PRAS40; Monoclonal Anti-PROLINE-RICH AKT SUBSTRATE, 40-KD
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 3 μg/mL Monoclonal Anti-AKT1S1 Clone: AKT1S1-3 (Cat. No. SAB4200256). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. PC3; 2. MG-63; 3. MDA-MB-231; 4. HEK-293; 5. HeLa
Immunoprecipitation Monoclonal Anti-AKT1S1 Clone: AKT1S1-3 (Cat. No. SAB4200256) was used to immunoprecipitate AKT1S1 from HEK-293 whole cells extract. Detection antibody: Polyclonal Anti-AKT1S1. Lanes 1.10 μg/mL antibody 2. Negative control: 10 μg/mL non-relevant antibody 3. Positive control: HEK-293 whole cell extract
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AKT1S1-3, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~40 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 2-8 μg/mL using whole extracts of human MDA-MB-231cells. | |
| UniProt accession no. | Q96B36  |
| Application: | Monoclonal Anti-AKT1S1 antibody is suitable for use in western blot (2-8 μg/mL using whole extracts of human MDA-MB-231cells) and immunoprecipitation. |
| Biochem/physiol Actions: | Akt1 substrate 1 (AKT1S1) is involved in the PI3KAkt/ protein kinase B (PKB) survival pathway. Phosphorylation of AKT1S1 by Akt and mammalian target of rapamycin complex 1 (mTORC1) disrupts the binding between mTORC1 and AKT1S1, relieves the inhibitory effect of AKT1S1 on mTORC1 activity, and leads to the binding of AKT1S1 to 14-3-3, a cytosolic anchor protein. The binding of AKT1S1 to 14-3-3 requires amino acids and insulin, which is partially inhibited by rapamycin. |
| General description: | AKT1S1 is a raptor-associated protein that interacts with mTORC1 and subsequently inhibits the mTORC1 signaling pathway. This raptor-binding protein also inhibits phosphorylation of S6K1 and cell growth. Expression of AKT1S1 has been implicated in breast and pulmonary carcinogenesis, as well as in mediating insulin sensitivity . Monoclonal Anti-AKT1S1 antibody is specific for human AKT1S1 (approx. 40 kDa). |
| General description: | Monoclonal Anti-AKT1S1 (mouse IgG1 isotype) is derived from the hybridoma AKT1S1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to the N-terminus of human AKT1S1. Akt1 substrate 1 (AKT1S1) is also named as proline-rich Akt-substrate of 40 kDa (PRAS40). The rapamycin-sensitive mammalian target of rapamycin complex 1 (mTORC1) consists of mTOR, raptor, mLST8 and AKT1S1, that controls protein translation. AKT1S1 contains a TOR signaling (TOS) motif that mediates its binding to mTORC1. |
| Immunogen: | fusion protein corresponding to the N-terminus of human AKT1S1. |
| Immunogen: | The corresponding sequence differs by a single amino acid in rat, and by 2 amino acids in mouse AKT1S1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |