Anti-PP2A, C subunit antibody, Mouse monoclonal
SIGMA/SAB4200266 - clone 7A6, purified from hybridoma cell culture
Synonym: MonoclonalAnti-PP2Ac; MonoclonalAnti-PP2CA; MonoclonalAnti-
Product Type: Chemical
Immunoblotting Whole extract of human A431 cells was separated on SDS-PAGE and probed with Monoclonal Anti-PP2AC Clone: 7A6 (Cat. No. SAB4200266). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody
Immunoblotting Whole extract of mouse NIH-3T3 cells was separated on SDS-PAGE and probed with Monoclonal Anti-PP2AC Clone: 7A6 (Cat. No. SAB4200266). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody
Immunoblotting Whole extract of rat RAT2 cells was separated on SDS-PAGE and probed with Monoclonal Anti-PP2AC Clone: 7A6 (Cat. No. SAB4200266). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7A6, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 36 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 1-2 μg/mL using whole extracts of mouse 3T3, rat Rat2 or human A431 cells. | |
| UniProt accession no. | P67775  |
| Application: | MonoclonalAnti-PP2A, C subunit antibody produced in mouse has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Monoclonal Anti-PP2Ac is specific for the α and β isoforms of PP2Ac in humans, rats and mice. The antibody has not been tested in other species for cross-reactivity. |
| Biochem/physiol Actions: | Protein phosphatase 2A (PP2A) is implicated in the negative control of cell growth and division. PP2Ac undergoes two post-translational modifications, phosphorylation and methylation, which are involved in the regulation of the enzyme activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PP2A is a serine/threonine phosphatase that downregulates the MAP kinase pathway and subsequently modulates mitogenic signaling. This phosphatase also mediates localization of Sgo1 at centromeres, Ras-1 activation, and chromosome segregation. The catalytic subunit of PP2A (PP2Ac) regulates the signaling pathway for glucose-stiμLated insulin secretion . |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |