Anti-Progerin antibody, Mouse monoclonal
SIGMA/SAB4200272 - clone 13A4, purified from hybridoma cell culture
Synonym: Anti-CDCD1; Anti-CDDC; Anti-CMD1A; Anti-CMT2B1; Anti-EMD2; Anti-FPL; Anti-FPLD; Anti-HGPS; Anti-IDC; Anti-LDP1; Anti-LFP; Anti-LMN1; Anti-LMNA; Anti-LMNC; Anti-LMNL1; Anti-Lamin A/C; Anti-PRO1; Anti-Renal carcinoma antigen NY-REN-32
Product Type: Chemical
Immunofluorescence HGPS patient skin fibroblasts (A-B) and control skin fibroblasts (C-D) were fixed and permeabilized with 4% paraformaldehyde followed by 0.15% Triton X-100 . Fixed cells were stained with Monoclonal Anti-Progerin Clone: 13A4 (Cat. No. SAB4200272). Counterstaining was done with DAPI. Images were generously provided by Dr. Giovanna Lattanzi, IGM-CNR, Bologna, Italy.
Immunoblotting Whole extract of HeLa cells overexpressing FLAG-tagged human Progerin was separated on SDS-PAGE and probed with Anti-Lamin A/C (Lane 1), Monoclonal Anti-FLAG Clone: M2 (Lane 2), and Monoclonal Anti-Progerin Clone: 13A4 (Cat. No. SAB4200272) (Lane 3). Image generously provided by Dr. Egon Ogris, University of Vienna.
Immunoprecipitation Anti-lamin A/C (non-murine) and Monoclonal Anti-Progerin Clone 13A4 (Cat. No. SAB4200272) were used to immunoprecipitate Progerin from HGPS patient skin fibroblasts and control fibroblasts whole extracts. Detection antibody: Monoclonal Anti-Progerin Clone: 13A4 (Cat. No. SAB4200272). The asterisk labels the IgG band. Images were generously provided by Dr. Giovanna Lattanzi, IGM-CNR, Bologna, Italy.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 13A4, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~70 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 2.5-5.0 μg/mL using whole extracts of HeLa cells overexpressing human progerin. | |
| UniProt accession no. | P02545   |
| Application: | Monoclonal Anti-Progerin antibody produced in mouse has been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Mutations in Lamin A have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy and progeroid syndromes (collectively termed laminopathies) and to premature aging (Hutchinson-Gilford progeria syndrome). Lamins A/C participate in maintaining nuclear integrity, modulation of gene expression, cell proliferation and apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Progerin (mouse IgG1 isotype) is derived from the hybridoma 13A4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. A-type lamins, lamin A and lamin C are products of a single gene, LMNA, which are produced by alternative splicing. Prelamin A, the precursor of Lamin A, is farnesylated at its carboxy terminus after synthesis and the last 18 amino acids, which contain the farnesyl group, are removed by the metalloprotease Zmpste24, producing the mature Lamin A. The LMNA gene codes for A-type lamin, lamin A/C. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |