Anti-Orai1 antibody, Mouse monoclonal
SIGMA/SAB4200273 - clone ORAI1-89, purified from hybridoma cell culture
Synonym: Monoclonal Anti-CRACM1; Monoclonal Anti-ORAI calcium release-activated calcium modulator 1; Monoclonal Anti-ORAT1; Monoclonal Anti-TMEM142A; Monoclonal Anti-calcium release-activated calcium channel protein 1; Monoclonal Anti-transmembrane protein 142A
Product Type: Chemical
Immunofluorescence Jurkat cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Monoclonal Anti-ORAI1 Clone: ORAI1-89 (Cat. No. SAB4200273). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of HEK-293T cells overexpressing human ORAI1 was separated on SDS-PAGE and probed with Monoclonal Anti-ORAI1 Clone: ORAI1-89 (Cat. No. SAB4200273). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.5 μg/mL antibody + ORAI1 immunizing peptide
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ORAI1-89, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~30 kDa (non-glycosylated Orai1) |
| antigen ~50 kDa (glycosylated Orai1) | |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human Orai1. | |
| UniProt accession no. | Q96D31   |
| Application: | Monoclonal Anti-Orai1 antibody has been used in • immunoblotting, • immunofluorescence • immunoprecipitation • proximity ligation assay. |
| Application: | Monoclonal Anti-Orai1 antibody produced in mouse has been used in proximity ligation assay. |
| Biochem/physiol Actions: | Orai1 essential for store-operated calcium entry in T cells and fibroblasts. Store-operated calcium entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels, following Ca2+ depletion from the endoplasmic reticulum (ER) stores, caused by stimulation of immune cells. This process is crucial for gene transcription, proliferation and cytokine release. Mutations in two highly conserved glutamate residues, E106 and E190, diminish Ca2+ influx and promote changes in ion selectivity, providing strong evidence that Orai is a pore subunit of the CRAC channel. Orai1 co-localizes with STIM1 (stromal interaction molecule 1) near the plasma membrane after store depletion. A point mutation in Orai1 is responsible for the genetic defect in store-operated Ca2+ entry and I-CRAC in cells of the hereditary severe combined immune deficiency (SCID) syndrome patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Orai1 (mouse IgG1 isotype) is derived from the hybridoma ORAI1-89 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide located near the C-terminus of human Orai1, conjugated to KLH. Orai1, also called Calcium release-activated calcium channel protein 1 (CRACM1), is an evolutionary conserved plasma membrane protein. Orai1 is predicted to contain four transmembrane domains with its N- and C-termini in the cell cytoplasm. |
| General description: | ORAI calcium release-activated calcium modulator 1 (Orai1), also known as transmembrane protein 142A (TMEM142A), is encoded by the gene mapped to human chromosome 12q24.31.{49}. The encoded protein contains four transmembrane helices with highly conserved glutamate residues in transmembrane helices 1 and 3. |
| Immunogen: | synthetic peptide located near the C-terminus of human Orai1, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |