Anti-Tensin 1 antibody produced in rabbit
SIGMA/SAB4200283 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-TNS1
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Tensin 1 antibody produced in Rabbit (Cat. No. SAB4200283) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Tensin 1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.
Immunohistochemistry Localization of tensin 1 at sarcomeric fibers in rat heart. Formalin-fixed paraffin-embedded tissue was stained with 10 μg/mL Anti-Tensin 1 (Cat. No. SAB4200283) followed by Goat Anti-Rabbit IgG, HRP conjugate (×20 magnification).
Immunofluorescence Cytoplasmic and focal adhesion localization of tensin 1 in chicken embryo fibroblast cells. Fixed cells were stained with 3 μg/mL Anti-Tensin 1 (Cat. No. SAB4200283) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Cell and tissue extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-Tensin 1 (Cat. No. SAB4200283), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. A10 2. Rat heart 3. HEK293-T overexpressing human tensin 1 4. HEK293-T non-transfected 5. A10: Antibody + immunizing tensin 1 peptide 6. HEK293-T overexpressing human tensin 1. Antibody + immunizing tensin 1 peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 225 kDa (rat) |
| antigen ~150 kDa (human) | |
| shipped in | dry ice |
| species reactivity | rat, human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat heart. |
| indirect immunofluorescence: 3-6 μg/mL using chicken embryo fibroblast cells. | |
| western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human tensin 1 | |
| UniProt accession no. | Q9HBL0  |
| Application: | Anti-Tensin 1 antibody may be used in immunofluorescence and immunohistochemistry. |
| Application: | Anti-Tensin 1 antibody produced in rabbit has been used in immunostaining and immunoblotting. |
| Biochem/physiol Actions: | Tensin 1 (TNS1) contributes to transforming growth factor (TGF)-β-induced myofibroblast differentiation and extracellular fibronectin and collagen matrix formation. Disruption in the gene and associated signaling might hinder tissue fibrosis. The encoded protein anchors to stress fibers by interacting with cytoplasmic tails of integrins and increases metastatic abilities of cancer cells. Tensin plays a vital role in activation of the c-Jun N-terminal kinases (JNK) and p38 pathways. |
| Biochem/physiol Actions: | Tensin 1 associates with actin, integrin, FAK, p130Cas, DLC-1 and protein phosphatase PP1 which affects cell polarization, migration and invasion. Tensin 1 undergoes extensive Ser/Thr and Tyr phosphorylation induced by extracellular matrix (ECM), various growth factors and oncogenes. In addition, tensin 1 is cleaved by calpain II, a focal adhesion protease, suggesting that tensin 1 plays important roles in organizing the actin cytoskeleton and mediating signal transduction. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tensin 1 (TNS1) is encoded by the gene mapped to human chromosome 2q35. The encoded protein is localized to focal adhesions and is mainly expressed in renal tubular epithelial cells (TECs). |
| Immunogen: | synthetic peptide corresponding to a sequence located in the internal region of human tensin1, conjugated to KLH. The corresponding sequence is identical in rat and mouse tensin1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |