Anti-Actin, Muscle specific antibody, Mouse monoclonal
SIGMA/SAB4200287 - clone HHF-35, purified from hybridoma cell culture
Product Type: Chemical
Immunoblotting Lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Mouse Anti-Actin Clone: HHF-35 (Cat. No. SAB4200287). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. COS-7; 3. CFB; 4. BHK; 5. NRK; 6. Rat heart; 7. 3T3; 8. L929
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HHF-35, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~42 kDa |
| shipped in | dry ice |
| species reactivity | rat, bovine, canine, chicken, human, mouse, hamster, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 2-4 μg/mL using A10 cells |
| immunohistochemistry: suitable | |
| western blot: 0.1-0.2 μg/mL using A10 cell extracts |
| Application: | The antibody may be used in various immunochemical techniques including, immunoblotting, immunocytochemistry and immunohistochemistry. |
| Biochem/physiol Actions: | Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Actin, muscle specific (mouse IgG1 isotype) is derived from the hybridoma HHF-35 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with SDS extracted protein fraction of human myocardium. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as six isoforms characterized by electrophoretic mobility and amino acid sequence analysis. |
| Immunogen: | SDS-extracted protein fraction of human myocardium. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |