Anti-Tensin 1 (internal) antibody produced in rabbit
SIGMA/SAB4200298 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-TNS; Anti-TNS1
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Tensin 1 (internal) antibody produced in Rabbit (Cat. No. SAB4200298) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Tensin 1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1 μg/mL.
Immunoblotting Tisue and cell extracts were separated on SDS-PAGE, blotted with 1 μg/mL Anti-Tensin 1 (internal) (Cat. No. SAB4200298), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat heart 2. A10 3. HEK293-T overexpressing human tensin 1 4. HEK293-T non-transfected 5. HEK293-T overexpressing human tensin 1. Antibody + tensin 1 immunizing peptide 6. Rat heart. Antibody + immunizing tensin 1 peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 225 kDa (rat) |
| antigen ~150 kDa (human) | |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK-293T cells overexpressing human tensin1. |
| western blot: 1.5-3 μg/mL using lysates of A10 cells | |
| UniProt accession no. | Q9HBL0  |
| Application: | Anti-Tensin 1 (internal) antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | Tensins play an important role in regulating cell shape and motility. Tensin 1 is involved in organizing the actin cytoskeleton and mediating signal transduction. TNS1 is implicated in myofibroblast differentiation. In addition, it also helps myofibroblasts to form nascent fibronectin and collagen matrix. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Tensin 1, also known as TNS1, is a member of tensin protein family. Tensins are multi domain proteins containing homologous C1, protein phosphatases (PTPases), C2, Src homology 2 (SH2) domains and pTyr-binding (PTB) domains. Tensin 1 mRNA is widely expressed in mouse embryo and various human adult tissues and is localized to focal adhesions. |
| Immunogen: | synthetic peptide corresponding to a sequence located in the internal region of human tensin1, conjugated to KLH. The corresponding sequence is identical in rat and mouse tensin1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |