Anti-Atg7 antibody, Mouse monoclonal
SIGMA/SAB4200304 - clone ATG7-13, purified from hybridoma cell culture
Synonym: Anti-
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-ATG7 Clone: ATG7-13 (Cat. No. SAB4200304). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T; 2. HeLa; 3. G361; 4. HepG2; 5. Jurkat; 6. NRK; 7. NIH-3T3
Immunoprecipitation Monoclonal Anti-ATG7 Clone:ATG7-13 (Cat. No. SAB4200304) was used to immunoprecipitate ATG7 from U87 whole cell extract. Detection antibody: Rabbit Anti-ATG7 (Cat. No. A2856). Lanes 1. Positive control: U87 whole cell extract 2. 10 μg antibody 3. 5 μg antibody 4. 2.5 μg antibody 5. Negative control: 5 μg non-relevant antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ATG7-13, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~75 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 2-4 μg/mL using whole extracts of HEK-293T or U87 cells | |
| UniProt accession no. | O95352  |
| Application: | Monoclonal Anti-Atg7 antibody produced in mouse has been used in: • immunofluorescence • immunohistochemistry (IHC) • western blotting • immunoprecipitation |
| Biochem/physiol Actions: | Atg7 can activate all three Atg8 mammalian homologues, (Golgi-associated ATPase enhancer of 16 kDa (GATE-16), GABA receptor-associated protein (GABARAP) and microtubule-associated protein light chain 3 (MAP-LC3). It forms a homodimer through the C-terminal region that is important for enzyme substrate interaction and E1-E2 complex formation. Atg7 was found to be essential for amino acid supply in neonates and starvation− induced bulk degradation of proteins and organelles in mice. Loss of Atg7 results in the accumulation of abnormal organelles and ubiquitin positive aggregates and leads to neurodegeneration. |
| Biochem/physiol Actions: | Autophagy related 7 (ATG7) plays a vital role in the regulation of adult hematopoietic stem cells (HSCs). ATG7 expression is elevated in eosinophilic esophagitis (EoE). Hence, this can be used as a potent tissue biomarker for diagnosis of EoE inflammation in pediatric patients. The encoded protein plays an essential role in autophagy vesicle elongation. In addition, it is also implicated in two vital ubiquitin-like reactions such as LC3 lipidation and Atg5/12 conjugation. Down regulated expression of Atg7 increases the sensitivity to chemotherapy and disables stroma-mediated chemoresistance in acute myeloid leukemia (AML). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Autophagy related 7 (ATG7) is encoded by the gene mapped to human chromosome 3p25.3. The encoded protein has ubiquitous expression. |
| General description: | Monoclonal Anti-Atg7 (mouse IgG1 isotype) is derived from the hybridoma ATG7-13 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence located near the N-terminus of human Atg7. |
| Immunogen: | synthetic peptide corresponding to a sequence located near the N-terminus of human Atg7, conjugated to KLH. The corresponding sequence is identical in mouse and rat Atg7. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |