Anti-O-GlcNAcase (OGA) (C-terminal region) antibody produced in rabbit
SIGMA/SAB4200311 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-
Product Type: Chemical
Immunoblotting Cell extracts were separated on SDS-PAGE, blotted with 2 μg/mL Anti-O-GlcNAcase (OGA) (C-terminal region) (Cat. No. SAB4200311), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. MCF7 2. MDCK 3. MCF7. Antibody + immunizing O-GlcNAcase peptide
Immunoprecipitation Anti-O-GlcNAcase (OGA) (C-terminal region) (Cat. No. SAB4200311), was used to immunoprecipitate O-GlcNAcase from MDCK cells. Detection antibody: Anti-O-GlcNAcase (OGA) (Cat. No. SAB4200267). Lanes 1. IP antibody: 3 μg 2. Negative control: without IP antibody 3. Negative control: without cell lysate
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~130 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 3-6 μg using MDCK cells. |
| western blot: 2-4 μg/mL using MCF7 cell extracts. | |
| UniProt accession no. | O60502  |
| Application: | Anti-O-GlcNAcase (OGA) (C-terminal region) antibody produced in rabbit has been used in: • Western blotting • Immunoprecipitation • Microarray analysis |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | β-N-acetylglucosaminidase (OGA) along with O-GlcNAc transferase (OGT) are key enzymes which regulate cycling O-linked N-acetylglucosamine. OGA is responsible for cleaving the modification from target proteins. OGA is also glycosylated by OGT and a regulatory feedback loop exists between these two enzymes. OGA and OGT have been found to strongly associate together in transcriptional co-repression complexes with histone deacetylases (HDACs). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The β-N-acetylglucosaminidase (OGA) gene encodes two alternatively spliced isoforms that are widely expressed in mammalian tissues. OGA (also known as O-GlcNAcase, MGEA5, NCOAT) belongs to the family of 84 glycoside hydrolases. The longer OGA form is a bifunctional nuclear/cytoplasmic enzyme that contains two distinct domains, an O-GlcNAcase domain at the N-terminus and a C-terminal putative histone acetyltransferase (HAT) domain. The shorter OGA form contains only the N-terminal O-GlcNAcase domain. |
| Immunogen: | synthetic peptide corresponding to a sequence near the C-terminus of human O-GlcNAcase (OGA), conjugated to KLH. The corresponding sequence is identical in human OGA isoform B, and highly conserved (single amino acid substitution) in rat and mouse OGA. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |