Anti-OS9 antibody, Mouse monoclonal
SIGMA/SAB4200317 - clone OS9-8, purified from hybridoma cell culture
Synonym: Anti-ERLEC2; Anti-Endoplasmic reticulum lectin 2; Anti-Endoplasmic reticulum lectin, ERLEC2; Anti-Erlectin 2; Anti-OS-9, amplified in osteosarcoma 9; Anti-Osteosarcoma amplified 9
Product Type: Chemical
Western Blotting HEK-293T cells overexpressing OS9 extract were separated on SDS-PAGE and probed with Monoclonal Anti-OS9 antibody produced in Mouse, Clone: OS9-8 (Cat. No. SAB4200317). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 2 μg/mL antibody 2. Negative Control
Immunoprecipitation Monoclonal Anti-OS9 Clone: OS9-8 (Cat. No. SAB4200317) was used to immunoprecipitate OS9 from NRK whole cell extract. Detection antibody: Rabbit Anti-OS9 (Cat. No. SAB4200021) Lanes 1. 20 μg antibody 2. 10 μg antibody 3. 5 μg antibody 4. Negative control: 10 μg non-relevant antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | OS9-8, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~97/88 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 2-4 μg/mL using HEK-293T cells overexpressing OS9. | |
| UniProt accession no. | Q13438  |
| Application: | Monoclonal Anti-OS9 antibody produced in mouse may be used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Amplified in osteosarcoma 9 (OS-9) is an endoplasmic reticulum (ER)-resident lectin involved in ER quality control. OS9 is upregulated in response to ER stress through activation of the inositol-requiring enzyme 1/X-box binding protein 1 (Ire1/Xbp1) pathway. OS9 associates both with components of the Endoplasmic-reticulum-ass |
| Biochem/physiol Actions: | Monoclonal Anti-OS9 recognizes human, rat and mouse OS9 isoforms 1 and 2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-OS9 (mouse IgG2b isotype) is derived from the hybridoma OS9-8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Amplified in osteosarcoma 9 (OS-9) is a N-glycosylated protein, mapped to human chromosome 12q13.3-q14.1. It is ubiquitously expressed in two isoforms OS-9.1 and OS-9.2. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of human OS9, conjugated to KLH. The corresponding sequence is identical in mouse and rat OS9. The antibody recognizes OS9 isoforms 1 and 2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |