Anti-phospho-CD3ζ (pTyr72) antibody produced in rabbit
SIGMA/SAB4200330 - IgG fraction of antiserum
Synonym: Anti-CD247; Anti-CD3H; Anti-CD3Q; Anti-CD3Z; Anti-T cell receptor T3 zeta chain; Anti-T3Z; Anti-TCRK; Anti-Tcrz
Product Type: Chemical
Immunoblotting Lysate of HEK-293T cells co-transfected with murine CD3#ζ and constitutively active Lck (Lck-Y505F) was separated on SDS-PAGE and probed with Rabbit Anti-phospho-CD3#ζ (pTyr72) (Cat. No. SAB4200330). The antibody was developed with Goat Anti-Rabbit IgG, peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate. Lanes 1. Antibody dilution 1:1,000 2. Antibody dilution 1:2,000 3. Antibody dilution 1:4000 4. Antibody dilution 1:1,000 + 10 MUg/mL immunizing phosphopeptide 5. Antibody dilution 1:1,000 + 10 MUg/mL non-phosphorylated form of the immunizing peptide
Immunoprecipitation Rabbit Anti-phospho-CD3#ζ (pTyr72) (Cat. No. SAB4200330) was used to immunoprecipitate CD3#ζ from lysate of HEK-293T cells co-transfected with murine CD3#ζ and constitutively active Lck (Lck-Y505F). Detection antibody: mouse Anti-CD3#ζ followed by Anti-Mouse IgG (Fc specific)-Peroxidase (Cat. No. A2554). Lanes 1. Negative control: IP antibody without lysate 2. IP antibody: 4 MUg antibody 3. IP antibody: 2 MUg antibody 4. IP antibody: 1 MUg antibody
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr72) |
| technique(s) | immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK. |
| western blot: 1:1,000-1:2,000 using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK. | |
| UniProt accession no. | P24161  |
| Application: | Anti-phospho-CD3ζ (pTyr72) antibody has been used in • immunoblotting • immunoprecipitation • western blotting |
| Biochem/physiol Actions: | T cell antigen receptor ζchain (CD3ζ) functions as a key signal transduction component of the T cell antigen receptor (TCR) complex. The encoded protein facilitates optimal effector T-cell function by enhancing receptor expression and signaling. Mutation in the gene increases the risk of susceptibility to systemic lupus erythematosus (SLE). Reduced expression of the gene has been observed in T-cells of cancer, lupus and chronic infectious diseases such as leprosy and tuberculosis patients. CD3ζ serves as a potent biomarker for determining progression and severity in patients with type 2 diabetes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | T cell antigen receptor ζ chain (CD3ζ), also known as CD247 gene, spanning 88kb of genomic DNA, is mapped to human chromosome 1q24.2. |
| General description: | The T cell antigen receptor-CD3 (TCR-CD3) multimeric complex is composed of TCRαβ, CD3γε, CD3δε and ζ ζ dimers. The CD3γ, CD3δ and CD3ε, each contain one immunoreceptor tyrosine-based activation motif (ITAM), whereas the ζ chain contains three ITAMs. |
| Immunogen: | synthetic phosphopeptide corresponding to residues surrounding Tyr72 of mouse CD3ζ, conjugated to KLH. The corresponding sequence differs by a single amino acid in human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |