Anti-phospho-CD3ζ (pTyr142) antibody produced in rabbit
SIGMA/SAB4200334 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-CD247; Anti-CD3H; Anti-CD3Q; Anti-CD3Z; Anti-T cell receptor T3 zeta chain; Anti-T3Z; Anti-TCRK; Anti-Tcrz
Product Type: Chemical
Immunoblotting Lysate of HEK-293T cells co-transfected with murine CD3#ζ and constitutively active Lck (Lck-Y505F) was separated on SDS-PAGE and probed with Rabbit Anti-phospho-CD3#ζ (pTyr142) (Cat. No. SAB4200334). The antibody was developed with Goat anti-Rabbit IgG, peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate. Lanes 1. Antibody: 0.5 MUg/mL 2. Antibody: 1 MUg/mL + 1MUg/mL non-phosphorylated form of the immunizing peptide 3. Antibody: 1 MUg/mL + 1 MUg/mL immunizing phosphopeptide.
Immunoprecipitation Rabbit Anti-phospho-CD3#ζ (pTyr142) (Cat. No. SAB4200334) was used to immunoprecipitate CD3#ζ from lysate of HEK-293T cells co-transfected with murine CD3#ζ and constitutively active Lck (Lck-Y505F). Detection antibody: mouse Anti-CD3#ζ followed by Anti-Mouse IgG (Fc specific)-Peroxidase (Cat. No. A2554). Lanes 1. Negative control: IP antibody without lysate 2. IP antibody: 4 MUg antibody 3. IP antibody: 2 MUg antibody 4. IP antibody: 1 MUg antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr142) |
| technique(s) | immunoprecipitation (IP): 1-2 μg using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK. |
| western blot: 0.5-1 μg/mL using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK. | |
| UniProt accession no. | P24161  |
| Application: | Anti-phospho-CD3ζ (pTyr142) antibody produced in rabbit has been used in several immunochemical techniques including immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Amongst the TCR subunits, the CD3 ζ chain plays a key role in regulating TCR assembly, transport, and cell surface expression. Down-regulation of TCR ζ chain expression associated with T cell dysfunction was described in various pathologies. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The TCR-CD3 multimeric complex is composed of TCRαβ, CD3γε and CD3δε and TCR‐ζ homodimer. The CD3γ, CD3δ and CD3ε, each contain one immunoreceptor tyrosine-based activation motif (ITAM), whereas the ζ chain contains three ITAMs. |
| Immunogen: | synthetic phosphopeptide corresponding to residues surrounding Tyr142 of mouse CD3ζ, conjugated to KLH. The corresponding sequence is identical in human CD3ζ. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |