Anti-Golph3 (N-terminal) antibody produced in rabbit
SIGMA/SAB4200341 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-GOPP1; Anti-GPP34; Anti-Golgi peripheral membrane protein 1, 34 kDa; Anti-Golgi phosphoprotein 3 (coat-protein); Anti-
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X100. Fixed cells were stained with 1 μg/mL Rabbit Anti-Golph3 (N-terminal) (Cat. No. SAB4200341). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-Golph3 (N-terminal) (Cat. No. SAB4200341). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa; 2. WI-38; 3. NCI-H1299; 4. LA4; 5. NIH-3T3; 6. PC-12
Immunoprecipitation Rabbit Anti-Golph3 (N-terminal) (Cat. No. SAB4200341) was used to immunoprecipitate Golph3 from HeLa whole cell extract. Detection antibody: Rabbit Anti-Golph3 (C-terminal) (Cat. No. SAB4200340). Lanes 1. 5 μg antibody 2. 10 μg antibody 3. Negative control: 10 μg non-relevant antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~34 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells |
| indirect immunofluorescence: 2.5-5.0 μg/mL using mouse 3T3 cells | |
| western blot: 1-2 μg/mL using whole extracts of human HeLa cells | |
| UniProt accession no. | Q9H4A6  |
| Application: | Anti-Golph3 (N-terminal) antibody produced in rabbit has been used in: • immunoprecipitation • immunofluorescence • immunoblotting |
| Biochem/physiol Actions: | Golph3 (Golgi phosphoprotein 3) is important for Golgi trafficking and morphology by interacting with the unconventional myosin MYO18A, linking Golgi membranes to the actin cytoskeleton. It is a phosphatidylinositol-4-ph |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Golph3 (Golgi phosphoprotein 3), also known as coat protein GPP34, is a peripheral membrane protein of the Golgi stack, that localizes to the trans-Golgi network. It is an oncogene that is commonly targeted for amplification in human cancer and in cancer cell lines. |
| Immunogen: | peptide corresponding to the N-terminal region of human Golph3, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and bovine. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |