Anti-SMAD7 antibody produced in rabbit
SIGMA/SAB4200345 - ~1.0 mg/mL, affinity isolated antibody
Synonym: SMAD7 Antibody - Anti-SMAD7 antibody produced in rabbit; Smad7 Antibody; Anti-MADH7; Anti-MADH8; Anti-SMAD family member 7
Product Type: Chemical
Immunofluorescence Human A549 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2.5 μg/mL Rabbit Anti-SMAD7 (Cat. No. SAB4200345). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of HEK-293T cells overexpressing human SMAD7 was separated on SDS-PAGE and probed with Rabbit Anti-SMAD7 (Cat. No. SAB4200345). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~46 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, bovine, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using human A549 cells. |
| western blot: 2-4 μg/mL using whole extracts of HEK-293 cells over-expressing human SMAD7 | |
| UniProt accession no. | O15105  |
| Application: | Anti-SMAD7 has been used in western blot analysis and immunofluorescence. |
| Biochem/physiol Actions: | SMAD7 (mothers against decapentaplegic homolog 7) is a modulator of TGFβ (transforming growth factorβ) signaling in immune cells which are associated with ulcerative colitis and inflammatory bowel syndrome. It plays a major role in the etiology of CRC (colorectal cancer). In addition, it also acts as a mediator of the negative feedback loop for both the TGFβ and BMP (bone morphogenetic proteins) signaling pathways. It is essential for the remodelling of pharyngeal artery and the enlargement of great vessel. In mouse, homozygous removal of Smad7 causes primarily fourth-related arch artery defects. Silencing of Smad7 in RCD (refractory coeliac disease) biopsy samples can decrease the expression of interleukin-6 and tumour necrosis factor-α. Polymorphism of this gene is associated with colorectal cancer. SMAD7 is an inhibitor of the TGFβ (transforming growth factor beta) /BMP (bone morphogenetic proteins) pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SMAD7 (mothers against decapentaplegic homolog 7) is a Tbx1 (T-box 1) interacting gene and is encoded by the gene mapped to human chromosome 18q21.1. |
| Immunogen: | peptide corresponding to an internal region of human SMAD7, conjugated to KLH. The corresponding sequence is identical in monkey, mouse, rat and bovine |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |