Anti-Nestin (C-terminal region) antibody produced in rabbit
SIGMA/SAB4200347 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-Nbla00170
Product Type: Chemical
Immunofluorescence Cytoskeletal localization of nestin in BHK cells. Fixed cells were stained with 2 μg/mL Anti-Nestin (C-terminal region) (Cat. No. SAB4200347) followed by Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunofluorescence Cytoskeletal co-localization of nestin and vimentin in BHK cells (confocal image, overlay). Fixed cells were stained with 2 μg/mL Anti-Nestin (C-terminal region) (Cat. No. SAB4200347) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (orange). Anti-vimentin (green). Nuclear staining with DAPI.
Immunofluorescence Membrane and nuclear localization of nestin in P19 cells. Fixed cells were stained with 1 μg/mL Anti-Nestin (C-terminal region) (Cat. No. SAB4200347) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Cell extracts were separated on SDS-PAGE, blotted with 2 μg/mL Anti-Nestin (C-terminal region) (Cat. No. SAB4200347), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. A10 cells 2. P19 cells 3. HEK293-T overexpressing rat nestin 4. HEK293-T non-transfected 5. HEK293-T overexpressing rat nestin + immunizing nestin peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~300 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells. |
| western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin. | |
| UniProt accession no. | P48681  |
| Application: | Anti-Nestin (C-terminal region) antibody has been used in • immunofluorescence • Proximity ligation assay • immunohistofluorescence staining • immunofluorescence labeling • immunoblotting |
| Application: | Anti-Nestin (C-terminal region) antibody produced in rabbit has been used in western blotting and immunostaining. |
| Biochem/physiol Actions: | Nestin (NES) functions as a modulator of proteins which are involved in cell growth. It also interacts with other proteins of the intermediate filament. NES has been studied as a marker of neural stem cells. |
| Biochem/physiol Actions: | Nestin expression is down-regulated and replaced by the expression of other intermediate filament proteins such as neurofilament and Glial fibrillary acidic protein (GFAP). Nestin expression is re-induced in the adult organism during pathological conditions, such as formation of glial scar after injury to the central nervous system (CNS) and in certain types of CNS tumors. Nestin has been shown to play an important role in the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis and is a potential target for Cyclin-dependent kinase 5 (cdk5) and p35 during development. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Nestin (NES) is a class VI cytoplasmic protein which is expressed in the intermediate filaments. It has a molecular weight of 177.4kDa. The gene encoding it is localized on human chromosome 1q23.1 and has four exons. |
| General description: | Nestin is expressed during early stages of development in the central nervous system (CNS) and in myogenesis. Nestin is found in neurogenic progenitor cells (NPCs) and has been used as a marker for neural progenitor cells. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminal region of human nestin, conjugated to KLH. The sequence is highly conserved (72% identity) in rat and mouse nestin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |