Anti-SMAD2 antibody produced in rabbit
SIGMA/SAB4200354 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-JV18; Anti-JV18-1; Anti-MADH2; Anti-MADR2; Anti-Mothers against decapentaplegic homolog 2; Anti-hMAD-2; Anti-hSMAD2
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 2.5 μg/mL Rabbit Anti-SMAD2 (Cat. No. SAB4200354). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting Whole extract of HEK-293T cells overexpressing human SMAD2 was separated on SDS-PAGE and probed with Rabbit Anti-SMAD2 (Cat. No. SAB4200354) The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~52 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using using human HeLa cells |
| western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD2 | |
| UniProt accession no. | Q15796  |
| Application: | Anti-SMAD2 antibody produced in rabbit may be used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Anti-SMAD2 recognizes human SMAD2. |
| Biochem/physiol Actions: | Members of the SMAD family are essential for transmitting signals from the transforming growth factor-β (TGFβ) to the nucleus, and thus regulate various cellular processes, including cell proliferation, apoptosis, and differentiation. Smad4 is essential for modulating the transactivation efficacy of the Smad complexes in the nucleus. SMAD2 is recruited to TGFβ receptors through its interaction with the SARA (SMAD anchor for receptor activation) protein. In response to TGFβ, SMAD2 is phosphorylated by TGFβ receptors. This phosphorylation induces the dissociation of SMAD2 from SARA and the association with the family member SMAD4.The association with SMAD4 is important for the translocation of SMAD2 into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SMAD2 belongs to the SMAD family of proteins. The members of this family contain two conserved domains, the N-terminal MH1 and C-terminal MH2, that are separated by a proline-rich linker region of varying length. The MH1 domain is implicated in the regulation of nuclear import and transcription by binding to DNA and interacting with nuclear proteins. The MH2 domain is involved in the modulation of Smad oligomerization and recognition by type I receptors and it associated with cytoplasmic adaptors and transcription factors. |
| General description: | The SMAD2 gene is located on the human chromosome at 18q21.1. |
| Immunogen: | peptide corresponding to an internal region of human SMAD2, conjugated to KLH. The corresponding sequence is identical in mouse, rat and bovine. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |