Anti-TGN46 antibody, Mouse monoclonal
SIGMA/SAB4200355 - clone TGN46-8, purified from hybridoma cell culture
Synonym: Anti-Trans-Golgi network protein, 46-kD
MDL Number: MFCD09265378
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Monoclonal Anti-TGN46 Clone: TGN46-8 (Cat. No. SAB4200355). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of HEK-293T cells overexpressing human TGN46 was separated on SDS-PAGE and probed with Monoclonal Anti-TGN46 Clone: TGN46-8 (Cat. No. SAB4200355). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + TGN46 immunizing peptide 3. 1 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TGN46-8, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | 80-100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using HeLa cells |
| western blot: 2-4 μg/mL using whole extracts of HEK-293T cells over-expressing human TGN46 | |
| UniProt accession no. | O43493  |
| Biochem/physiol Actions: | Trans-Golgi network integral membrane protein 2 (TGOLN2) cycles between the trans-Golgi network (TGN) and the cell surface via an early endosomal compartment. This movement is mediated by a tyrosine-based tetra peptide signal (SDYQRL) in the cytoplasmic domain. It plays an important role in the formation of exocytic vesicles at the TGN by functioning as a receptor for complexes of a cytoplasmic protein known as p62 and one GTP-binding protein. The cytoplasmic domain of TGOLN2 binds to the complex and is essential for budding process. It mediates the coupling of the segregation of secretory proteins to the budding of exocytic vesicles. The cytosolic domain of TGOLN2 interacts with AP2 clathrin adaptor complexes and also with the coiled coil region of a protein called neurabin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Trans-Golgi network integral membrane protein 2 (TGOLN2), also known as trans-Golgi network 46 (TGN46), is encoded by the gene mapped to human chromosome 2p11.2. It is a heterodimeric type I integral membrane protein. TGOLN2 has a highly conserved N terminus, which consists of a signal peptide. The C terminus possesses a lumenal domain, a membrane spanning region and cytoplasmic tail. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of human TGN46, conjugated to KLH. The corresponding sequence is identical in monkey and differs by 3 amino acids in rat and mouse TGN46. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |