Anti-Profilin 1 antibody, Mouse monoclonal
SIGMA/SAB4200357 - clone Profilin 1-3, purified from hybridoma cell culture
Synonym: Anti-PFN1
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-Profilin1 Clone: Profilin1-3 (Cat. No. SAB4200357). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HepG2 2. L8 3. NRK 4. 3T3 5. Hepa 1-6 6. BMGE 7. MDCK
Immunoprecipitation Monoclonal Anti-Profilin1 Clone: Profilin1-3 (Cat. No. SAB4200357) was used to immunoprecipitate Profilin1 from HeLa whole cell extract. Detection antibody: Rabbit Anti-Profilin (C-terminal) (Cat. No. P7624). Lanes 1. Positive control: HeLa whole cell extract 2. 8 μg antibody 3. 4 μg antibody 4. 2 μg antibody 5. Negative control: 4 μg non-relevant antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | Profilin 1-3, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~15 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, bovine, human, mouse, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 1-2 μg/mL using whole extracts of HeLa or NRK cells | |
| UniProt accession no. | P07737  |
| Application: | Monoclonal Anti-Profilin 1 antibody produced in mouse has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Profilin-1 (Pfn1) is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Profilin 1 is a potent regulator of actin filament dynamics. It is suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over expressed in breast cancer cells. Deletion of the profilin 1 gene leads to an embryonic lethal phenotype. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Profilin 1 (mouse IgG1 isotype) is derived from the hybridoma Profilin1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence located near the C-terminus of human profiling 1, conjugated to keyhole limpet hemocyanin (KLH). Profilin-1 (Pfn1), a major actin-regulatory protein is mapped to human chromosome17p13. It is the most ubiquitous and abundant protein and is highly expressed throughout development and adulthood in most tissues including brain. |
| Immunogen: | synthetic peptide corresponding to a sequence located near the C-terminus of human profilin 1, conjugated to KLH. The corresponding sequence is identical in mouse and rat profilin 1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |