Anti-LC3B antibody, Mouse monoclonal
SIGMA/SAB4200361 - clone LC3B-6, purified from hybridoma cell culture
Synonym: Monoclonal Anti-ATG8F; Monoclonal Anti-LC3; Monoclonal Anti-MAP1A/1BLC3; Monoclonal Anti-MAP1LC3B; Monoclonal Anti-
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing human LC3B was separated on SDS-PAGE and probed with Monoclonal Anti-LC3B Clone: LC3B-6 (Cat. No. SAB4200361). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + LC3B immunizing peptide
Immunoprecipitation Monoclonal Anti-LC3B Clone: LC3B-6 (Cat. No. SAB4200361) was used to immunoprecipitate LC3B from U-87 whole cell extract. Detection antibody: Rabbit Anti-LC3B (Cat. No. L7543). Lanes 1. Positive control: U-87 cells extract 2. 5 μg antibody 3. 10 μg antibody 4. 20 μg antibody 5. Negative control: without IP antibody 6. Negative control: 10 μg non-relevant antibody 7. Negative control: without cell extract
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | LC3B-6, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~18/16 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-20 μg using lysates of human U-87 cells. |
| western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human LC3B. | |
| UniProt accession no. | Q9GZQ8  |
| Application: | Anti-LC3B antibody produced in rabbit has been used in: • western blotting • immunofluorescence • confocal laser microscopy • immunoprecipitation |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Biochem/physiol Actions: | MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I. Anti-LC3B antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma LC3B-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the N-terminal region of human LC3B. |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of human LC3B, conjugated to KLH. The corresponding sequence differs by one amino-acid in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |