Anti-PIWIL1 antibody, Mouse monoclonal
SIGMA/SAB4200365 - clone PIWIL1-2, purified from hybridoma cell culture
Synonym: Monoclonal Anti-PIWIL1 antibody produced in mouse; Monoclonal Anti-HIWI; Monoclonal Anti-MIWI; Monoclonal Anti-PIWI; Monoclonal Anti-piwi-like 1
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded mouse testis sections with 10 μg/mL Monoclonal Anti PIWIL1 Clone: PIWIL1-2 (Cat. No. SAB4200365) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Whole extract of HEK-293T cells overexpressing human PIWIL1 was separated on SDS-PAGE and probed with Monoclonal Anti-PIWIL1 Clone: PIWIL1-2 (Cat. No. SAB4200365). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2.5 μg/mL antibody 2. 5 μg/mL antibody 3. 5 μg/mL antibody + PIWIL1 immunizing peptide
Immunoprecipitation Monoclonal Anti-PIWIL1 Clone: PIWIL1-2 (Cat. No. SAB4200365) was used to immunoprecipitate PIWIL1 from mouse testis whole cell extract. Detection antibody: Rabbit Anti-PIWIL1 (Internal Region) (Cat. No. SAB4200110). Lanes 1. 5 μg antibody 2. 10 μg antibody 3. 20 μg antibody 4. Negative control: without IP antibody 5. Negative control: 10 μg non-relevant antibody 6. Negative control: without cell extract
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PIWIL1-2, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~95 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin® Peroxidase staining system. |
| immunoprecipitation (IP): suitable | |
| western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1. | |
| UniProt accession no. | Q96J94  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunoprecipitation (1 paper) |
| Application: | Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in: • immunofluorescence • immunoprecipitation • flow cytometry analysis29} • immunoblotting • immunohistochemistry |
| Biochem/physiol Actions: | Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33. |
| Immunogen: | synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |