Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse
SIGMA/SAB4200369 - clone R2.5254.2, purified from hybridoma cell culture
Synonym: Anti-CD112; Anti-HVEB; Anti-PRR2; Anti-PVRL2; Anti-PVRR2; Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)
Product Type: Chemical
Immunoblotting Cell lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-Nectin-2/PVRL2 Clone: R2.5254.2 (Cat. No. SAB4200369). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. COS-7 2. GH3 3. LA4 4. WiDr
Flow Cytometry 1×106 MDA-MB-231 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton X-100. Fixed cells were labeled with Mouse Anti-NECTIN2/PVRL2 Clone: R2.525.2 (Cat. No. SAB4200369) 5 μg//test followed by Rabbit Anti-Mouse IgG, FITC conjugate.Green histogram: negative control without first antibody; Purple histogram: antibody specific staining.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | R2.5254.2, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~72 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, monkey, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells |
| immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells | |
| indirect ELISA: suitable | |
| western blot: suitable |
| Application: | Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • immunocytochemistry • flow cytometry |
| Biochem/physiol Actions: | Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC). |
| Biochem/physiol Actions: | The antibody has been reported to block cell adhesion and herpes virus entry. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells. |
| General description: | Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain. |
| Immunogen: | 3T3 cells expressing human Nectin-2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |