Anti-SMAD6 (C-terminal) antibody produced in rabbit
SIGMA/SAB4200383 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-MADH6; Anti-MADH7; Anti-SMAD family member 6
Product Type: Chemical
Immunofluorescence Mouse NIH-3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-SMAD6 (C-terminal) (Cat. No. SAB4200383). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting Whole extract of HEK-293T cells overexpressing human SMAD6 (C-terminal) was separated on SDS-PAGE and probed with Rabbit Anti-SMAD6 (C-terminal) (Cat. No. SAB4200383). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. 0.5 μg/mL antibody 4. 2 μg/mL antibody + SMAD6 immunizing peptide
Immunoprecipitation Rabbit Anti-SMAD6 (C-terminal) (Cat. No. SAB4200383) was used to immunoprecipitate SMAD6 from HEK-293T overexpressing human SMAD6. Detection antibody: Rabbit Anti-SMAD6 (N-terminal) (Cat. No. SAB4200384). Lanes 1. Negative control: without cell extract 2. Negative control: without immunoprecipitating antibody 3. 2.5 μg antibody. 4. 5 μg antibody. 5. Positive control: HEK-293T overexpressing SMAD6
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over-expressing human SMAD6. |
| indirect immunofluorescence: 10-20 μg/mL using mouse 3T3 cells. | |
| western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD6. | |
| UniProt accession no. | O43541  |
| Biochem/physiol Actions: | SMAD family member 6 (SMAD6) induces endocytosis and negatively regulates apoptosis by inhibiting transforming growth factor-β (TGF-β) signaling pathway. In addition, it also indirectly regulates stemness by inhibiting erythropoiesis in cord blood hematopoietic stem cells (HSCs). Mutation in the gene leads to congenital cardiovascular malformation (CVM). Overexpression or genetic variation of SMAD6 is associated with the development of ovarian cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SMAD family member 6 (SMAD6) is encoded by the gene mapped to human chromosome 15q22.31. The encoded protein is localized in both nuclei and cytoplasm and is expressed in variety of human tissues, including ovary. SMAD6 consists of MAD homology 2 (MH2) domain, involved in protein-protein interaction. |
| Immunogen: | peptide corresponding to the C-terminal region of human SMAD6, conjugated to KLH. The corresponding sequence is identical in rat, mouse and bovine. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |