Anti-SMAD6 (N-terminal) antibody produced in rabbit
SIGMA/SAB4200384 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-MADH6; Anti-MADH7; Anti-SMAD family member 6
Product Type: Chemical
Immunofluorescence Mouse NIH-3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-SMAD6 (N-terminal) (Cat. No. SAB4200384). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting Whole extract of HEK-293T cells overexpressing human SMAD-6 (N-terminal) was separated on SDS-PAGE and probed with Rabbit Anti-SMAD6 (N-terminal) (Cat. No. SAB4200384). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. 0.5 μg/mL antibody 4. 2 μg/mL antibody + SMAD6 (N-terminal) immunizing peptide
Immunoprecipitation Rabbit Anti-SMAD6 (N-terminal) (Cat. No. SAB4200384) was used to immunoprecipitate SMAD6 from HEK-293T overexpressing human SMAD6. Detection antibody: Rabbit Anti-SMAD6 (C-terminal) (Cat. No. SAB4200383). Lanes 1. 5 μg antibody 2. 10 μg/mL antibody 3. Negative control: without immunoprecipitating antibody 4. Negative control: without cell extract 5. Positive control: HEK-293T overexpressing SMAD6
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over-expressing human SMAD6 |
| indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells | |
| western blot: 1-2 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD6 | |
| UniProt accession no. | O43541  |
| Application: | Anti-SMAD6 (N-terminal) antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | SMAD6 plays an important role in transforming growth factor-β (TGF-β) pathway. It regulates cell proliferation, apoptosis and differentiation. SMAD6 is a key negative regulator of bone morphogenetic protein (BMP) signaling in the cytoplasm and in the nucleus. SMAD6 inhibits SMAD signaling in the nucleus by interacting with transcriptional repressors such as HOXC-8, class I histone deacetylases (HDACs) and transcriptional corepressor C-terminal binding protein (CtBp). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SMAD6 is a member of inhibitory Smads (I-Smad) family. It consists of two conserved domains, the N-terminal MH1 and C-terminal MH2, that are separated by a proline-rich linker region of varying length. |
| Immunogen: | peptide corresponding to the N-terminal region of human SMAD6, conjugated to KLH. The corresponding sequence is identical in rat and differs by a single amino acid in mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |