Anti-Nestin (C-terminal) antibody produced in rabbit
SIGMA/SAB4200394 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-NES
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Nestin (C-terminal) antibody produced in Rabbit (Cat. No. SAB4200394) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Nestin (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 2 μg/mL.
Immunofluorescence Cytoskeletal localization of nestin in BHK cells. Fixed cells were stained with 2 μg/mL Anti-Nestin (C-terminal) (Cat. No. SAB4200394) followed by Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunofluorescence Cytoskeletal co-localization of nestin and vimentin in BHK cells (confocal image, overlay). Fixed cells were stained with 2 μg/mL Anti-Nestin (C-terminal) (Cat. No. SAB4200394) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (orange), and Anti-vimentin (green). Nuclear staining with DAPI.
Immunofluorescence Membrane and nuclear localization of nestin in P19 cells. Fixed cells were stained with 3 μg/mL Anti-Nestin (C-terminal) (Cat. No. SAB4200394) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Cell extracts were separated on SDS-PAGE, blotted with 2 μg/mL Anti-Nestin (C-terminal) (Cat. No. SAB4200394), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. P19 cells 2. HEK293-T overexpressing rat nestin 3. HEK293-T non-transfected 4. HEK293-T overexpressing rat nestin. Antibody + immunizing nestin peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~300 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells |
| western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin. | |
| UniProt accession no. | P21263  |
| Application: | Anti-Nestin (C-terminal) antibody has been used in immunostaining. |
| Biochem/physiol Actions: | Nestin/NES plays a major role in the modulation of assembly and disassembly of intermediate filaments, which in turn help in the remodeling of the cell along with other structural proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Nestin/NES belongs to the intermediate filament (IF) protein family. Its expression is found in immature neuroepithelial and progenitor cell types. This 240 kDa protein contains the shortest head domain (N-terminus) and a long tail domain (C-terminus). Nestin is mapped to human chromosome 1q23.1. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C- terminus of mouse nestin, conjugated to KLH. The sequence is identical in rat nestin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |