Anti-TFRC antibody produced in rabbit
SIGMA/SAB4200398 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-CD71; Anti-TFR; Anti-TFR1; Anti-TR; Anti-TRFR; Anti-Transferrin receptor (p90, CD71); Anti-Transferrin receptor protein 1; Anti-p90; AntiT9
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Rabbit Anti-TFRC (Cat. No. SAB4200398). The antibody was developed using Goat Anti-Rabbit IgG, Atto-488 conjugate.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 5 μg/mL Rabbit Anti-TFRC (Cat. No. SAB4200398). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa; 2. U-2-OS; 3. Jurkat; 4. EL4; 5. RAW-264; 6. A9; 7. A20; 8. Mouse spleen
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~90 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 10-20 μg/mL using rat NRK cells |
| western blot: 2.5-5.0 μg/mL using whole extracts of human U-2-OS and mouse Neuro-2a cells | |
| UniProt accession no. | P02786  |
| Application: | Anti-TFRC antibody produced in rabbit has been used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Transferrin receptor (TFRC) mediates cellular iron uptake. It binds iron bound transferrin on the cell surface, and the receptor ligand complex undergoes endocytosis via clathrin coated pits. Endosomal acidification leads to iron release, which is transported to the cytosol or to the mitochondria in erythroid cells. The apotransferrin receptor complex is then recycled to the cell surface, where the affinity of apotransferrin for TFRC drops, resulting in its dissociation. TFRC is necessary for development of erythrocytes and the nervous system. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Transferrin receptor (TFRC), also known as p90, CD71, TFR and TFR1, is a transmembrane glycoprotein. It is composed of two disulphide-bonded sub-units, contains three N-linked glycan units and is post-translationally modified with phosphate and fatty acyl groups. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human TFRC, conjugated to KLH |
| Physical form: | solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |