Anti-WIPI-2 antibody produced in rabbit
SIGMA/SAB4200399 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-ATG18B; Anti-Atg21; Anti-CGI-50; Anti-WIPI2, WD repeat domain, phosphoinositide interacting 2
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing human WIPI2 was separated on SDS-PAGE and probed with Rabbit Anti-WIPI2 (Cat. No. SAB4200399). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + WIPI2 immunizing peptide 3. 1 μg/mL antibody
Immunoblotting Whole extract of HEK-293T cells was separated on SDS-PAGE and probed with Rabbit Anti-WIPI2 (Cat. No. SAB4200399). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + WIPI2 immunizing peptide
Immunoprecipitation Rabbit Anti-WIPI2 (Cat. No. SAB4200399) was used to immunoprecipitate WIPI2 from an NRK whole cell extract. Detection antibody: Rabbit Anti-WIPI2 (C-terminal) (Cat. No. SAB4200400). Lanes 1. Positive control: NRK whole cell extract 2. 10 μg antibody 3. 5 μg antibody 4. 2.5 μg antibody 5. Negative control: 10 μg non-relevant antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~49 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells. |
| western blot: 1-2 μg/mL using using whole extracts of HEK-293T cells over-expressing mouse WIPI-2. | |
| UniProt accession no. | Q9Y4P8  |
| Application: | Anti-WIPI-2 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-WIPI-2 recognizes human, rat, and mouse WIPI-2. |
| Biochem/physiol Actions: | WIPI-2 (WD repeat domain, phosphoinositide-interact |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The WIPI2 gene is mapped on the human chromosome at 7p22.1. WIPI2 has a 7-bladed propeller structure and contains a conserved motif. |
| General description: | WD repeat domain, phosphoinositide-interact |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |