Anti-WIPI-2 (C-terminal) antibody produced in rabbit
SIGMA/SAB4200400 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-ATG18B; Anti-Atg21; Anti-CGI-50; Anti-WD repeat domain, phosphoinositide interacting 2; Anti-WIPI2
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-WIPI2 (C-terminal) (Cat. No. SAB4200400). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HEK-293T 2. G361 3. SHSY-5Y 4. HCT-116 5. NRK 6. NIH-3T3
Immunoprecipitation Rabbit Anti-WIPI2 (C-terminal) (Cat. No. SAB4200400) was used to immunoprecipitate WIPI2 from an NRK whole cell extract. Detection antibody: Rabbit Anti-WIPI2 (C-terminal) (Cat. No. SAB4200400). Lanes 1. Positive control: NRK whole cell extract 2. 5 μg antibody 3. 2.5 μg antibody 4. Negative control: 5 μg non-relevant antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~49 kDa |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells. |
| western blot: 2-4 μg/mL using whole extracts of human G-361 and NIH-3T3 cells. | |
| UniProt accession no. | Q9Y4P8   |
| Application: | Anti-WIPI-2 (C-terminal) antibody produced in rabbit may be used in various immunochemical techniques including • immunoblotting • immunoprecipitation • immunostaining. |
| Biochem/physiol Actions: | WIPI subfamily proteins acts as a key component of many essential biological functions such as signal transduction, transcription regulation and apoptosis. WIPI-2 is a phosphatidylinositol-3-ph |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | WIPI-2 belongs to the WIPI subfamily of WD40 repeat proteins. It is widely expressed in a variety of cell lines. The protein is characterized with a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. It is a membrane constituent of autophagosomes and the plasma membrane. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of human WIPI-2, conjugated to KLH. The corresponding sequence is identical in mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |