Anti-EXOSC8 (N-terminal) antibody produced in rabbit
SIGMA/SAB4200403 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anit-CIP3; Anit-Exosome component 8,p9; Anti-EAP2; Anti-OIP2; Anti-Opa interacting protein 2; Anti-RP11-421P11.3; Anti-RRP43; Anti-Ribosomal RNA-processing protein 43; Anti-Rrp43p; AntiCBP-interacting protein 3
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 5 μg/mL Rabbit Anti-EXOSC8 (N-Terminal) (Cat. No. SAB4200403). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HEK-293T overexpressing EXOSC8 2. A549 3. HepG2 4. HCT116 5. HL-60 6. HeLa 7. HEK-293T 8. COS 7 9. PC12
Immunoprecipitation Rabbit Anti-EXOSC8 (N-Terminal) (Cat. No. SAB4200403) was used to immunoprecipitate EXOSC8 from HEK-293T whole cell extract. Detection antibody: Rabbit Anti-EXOSC8 (C-Terminal) (Cat. No. SAB4200404). Lanes 1. 5 μg antibody 2. 2.5 μg antibody 3. 1.2 μg antibody 4. Negative control: without 1st antibody 5. Negative control: without cell extract 6. HEK-293T total cell extract
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~34 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1-2 μg using human HEK-293T cell lysate |
| western blot: 1-2 μg/mL using whole extracts of mouse A20 and rat PC12 cells | |
| UniProt accession no. | Q96B26  |
| Application: | Anti-EXOSC8 (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-EXOSC8 (N-terminal) recognizes human, mouse and rat EXOSC8. |
| Biochem/physiol Actions: | Exosome component 8 (EXOSC8) interacts with AU-rich element (ARE)-containing mRNAs and its deficiency halts growth in yeast. Mutations in the EXOSC8 gene is implicated in Ponto-cerebellar hypoplasia. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Exosome component 8 (EXOSC8) or RRP43, a 3′ to 5′ exoribonuclease, is a component of the eukaryotic exosome (a 400 kDa complex). EXOSC8 is part of the hexameric ring, along with EXOSC4-7 and EXOSC9. This is further capped by three RNA binding subunits (EXOSC1-EXOSC3). EXOSC8 gene is mapped to human chromosome 13q13.3 and is localized in nucleus and cytoplasm. |
| Immunogen: | peptide corresponding to the N-terminal region of human EXOSC8, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |