Anti-NEZHA antibody, Mouse monoclonal
SIGMA/SAB4200415 - clone NEZHA-1, purified from hybridoma cell culture
Synonym: Anti-CAMSAP3; Anti-Calmodulin regulated spectrin-associated protein family member 3; Anti-KIAA; Monoclonal Anti-NEZHA antibody produced in mouse
Product Type: Chemical
Immunofluorescence MDCK cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-NEZHA Clone: NEZHA-1 (Cat. No. SAB4200415). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoprecipitation Monoclonal Anti-NEZHA Clone: NEZHA-1 (Cat. No. SAB4200415) was used to immunoprecipitate NEZHA from a whole extract of HEK-293T cells overexpressing human NEZHA. Detection antibody: Rabbit Anti-NEZHA (C-terminal) (Cat. No. SAB4200171). Lanes 1. 5 μg antibody 2. 10 μg antibody 3. Negative control: 10 μg non-relevant antibody 4. Negative control: no cell extract 5. Whole extract of HEK-293T cells overexpressing human NEZHA
Immunoblotting Whole extract of SW480 was separated on SDS-PAGE and probed with Monoclonal Anti-NEZHA Clone: NEZHA-1 (Cat. No. SAB4200415). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2.5 μg/mL antibody 2. 5 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | NEZHA-1, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~150 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 2.5-5.0 μg/mL using whole extracts of human SW480 cells | |
| UniProt accession no. | Q9P1Y5  |
| Application: | Monoclonal Anti-NEZHA antibody produced in mouse has been used in immunofluorescence staining. |
| Application: | Monoclonal Anti-NEZHA antibody produced in mouse has been used in: • immunofluorescence • immunoprecipitation • immunofluorescence • mass-spectrometry-based analysis of a streptavidin pull-down assay • immunostaining |
| Biochem/physiol Actions: | CAMSAP3 (calmodulin-regulated spectrin-associated protein 3)/ NEZHA modulates the minus-end dynamics of microtubules. It helps to maintain neuronal polarity. Absence of this gene results in the formation of supernumerary axon. |
| Biochem/physiol Actions: | NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) facilitates translocation of Golgi vesicles in epithelial cells. In addition, it also helps in positioning the apical-to-basal polarity of microtubules in epithelial cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-NEZHA (mouse IgG1 isotype) is derived from the hybridoma NEZHA-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. Nezha (a deity in Chinese mythology) also known as KIAA1543 and calmodulin-regulated spectrin associated protein, is a 1276 amino acid protein that contains one calponin homology (CH) and two coiled coil (CC1 and 2) domains, as well as one DUF1781 (DUF) domain of unknown function. NEZHA is mapped to human chromosome 19p13. |
| General description: | NEZHA/ calmodulin regulated spectrin associated protein family member 3 (CAMSAP3) contains a conserved domain called cholecystokinin (CKK) domain at carboxy-terminal, as well as several coiled-coil regions and an calponin homology domain at amino-terminal. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey and dog NEZHA. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |