Anti-FUS antibody produced in rabbit
SIGMA/SAB4200454 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-ALS6; Anti-CHOP; Anti-FUS-CHOP; Anti-FUS1; Anti-TLS; Anti-TLS/CHOP; Anti-hnRNP-P2
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Human colon sections stained with 5 μg/mL Anti-FUS antibody produced in Rabbit (Cat. No.SAB4200454) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 5 μg/mL Anti-FUS (internal) antibody produced in Rabbit (Cat. No. SAB4200455) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-FUS antibodies, SAB4200454 (A) and SAB4200455 (B), target different regions of FUS show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Localization of FUS in human colon. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL. Anti-FUS (Cat. No. SAB4200454) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunohistochemistry Localization of FUS in rat colon. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL. Anti-FUS (Cat. No. SAB4200454) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunofluorescence Nuclear localization of FUS in HeLa cells. Fixed cells were stained with 2.5 μg/mL Anti-FUS (Cat. No. SAB4200454) followed by Goat Anti-Rabbit IgG, FITC conjugate (×40 magnification). Actin staining with phalloidin rhodamine conjugate.
Immunoblotting Cell extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-FUS (Cat. No. SAB4200454) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Mouse brain 2. Rat brain 3. K562 4. Jurkat 5. Jurkat + immunizing FUS peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~68 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat colon. |
| indirect immunofluorescence: 2.5-5 μg/mL using HeLa cells. | |
| western blot: 1.5-3.0 μg/mL using using lysates of Jurkat cells. | |
| UniProt accession no. | P35637  |
| Application: | Anti-FUS antibody produced in rabbit has been used in: • immunohistochemistry • immunoblotting • immunofluorescence |
| Biochem/physiol Actions: | Anti-FUS specifically recognizes human and rat FUS. |
| Biochem/physiol Actions: | Fused in sarcoma (FUS) plays regulatory roles in transcription, RNA splicing and transport and is implicated in multiple diseases. FUS also called translocation in liposarcoma or Tumor lysis syndrome (TLS), plays a key role in DNA repair and transcriptional regulation. Chromosomal translocation of FUS/TLS is found in human cancers and results in the production of oncogenic FUS fusion proteins. FUS is a component of inclusion bodies in patients with Huntington′s disease (HD) and spinocerebellar ataxias (SCA1) and SCA3. Mutations in the FUS gene have been identified in amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration (FLTD) and familial amyotrophic lateral sclerosis (FALS). The majority of the FUS mutations have been identified in the C-terminal nuclear localization signal (NLS). Pathological FUS inclusions are mostly found in the cytosol of neurons and glial cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fused in sarcoma (FUS) is a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complex is a DNA/ RNA binding protein. FUS gene is mapped to human chromosome 16p11.2 and is located predominantly in the nucleus. |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of human FUS isoform 1, conjugated to KLH. The corresponding sequence is identical in human FUS isoforms 2 and 3, and highly conserved (single amino acid insertion) in mouse and rat FUS. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |