Anti-Desmoglein 2 (DSG2) antibody, Mouse monoclonal
SIGMA/SAB4200466 - clone AH12.2, purified from hybridoma cell culture
Synonym: Monoclonal Anti-ARVC10; Monoclonal Anti-ARVD10; Monoclonal Anti-CDHF5; Monoclonal Anti-CMD1BB; Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse; Monoclonal Anti-HDGC; Monoclonal Anti-desmoglein 2
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 5 μg/mL Mouse Anti-Desmoglein 2 Clone: AH12.2 (Cat. No. SAB4200466). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting HeLa cell extract was separated on SDS-PAGE and probed with Mouse Anti-Desmoglein 2 Clone: AH12.2 (Cat. No. SAB4200466). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. Negative control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AH12.2, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| isotype | IgG1 |
| mol wt | antigen ~150 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells |
| western blot: 0.5-1.0 μg/mL using HeLa total cell extracts |
| Application: | Monoclonal Anti-Desmoglein 2 (DSG2) antibody produced in mouse has been used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Desmogleins play an important role in the formation of desmosomes, cell to-cell adhesions, often found in both simple and squamous epithelium. These adhesions are important for ensuring tissue integrity and regulation of paracellular movement of solutes and restricting access of luminal pathogens to underlying tissue compartments. Desmoglein-2 (DSG2) has been shown to regulate numerous cellular processes, including proliferation and apoptosis. Intracellular fragments of DSG2 promote apoptosis in colonic epithelial cells. Mutations in this gene is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Desmoglein 2 (DSG2) (mouse IgG1 isotype) is derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells. Desmogleins are a family of cadherin proteins. There are four distinct desmoglein genes (DSG1-DSG4), each differentially expressed depending upon the type of tissue and the state of differentiation. Desmoglein-2 (DSG2) is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis and heart. |
| Immunogen: | derived from the hybridoma AH12.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with lipid raft enriched preparations of human T84 intestinal epithelial cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |