Anti-JAM-A antibody, Mouse monoclonal
SIGMA/SAB4200468 - clone J10.4, purified from hybridoma cell culture
Synonym: Monoclonal Anti-CD321; Monoclonal Anti-F11 receptor; Monoclonal Anti-F11R; Monoclonal Anti-JAM; Monoclonal Anti-JAM1; Monoclonal Anti-JAMA; Monoclonal Anti-JCAM; Monoclonal Anti-KAT; Monoclonal Anti-PAM-1
Product Type: Chemical
Immunofluorescence HepG2 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 100 μg/mL Mouse Anti-JAM-A Clone: J10.4 (Cat. No. SAB4200468). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence A431 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 100 μg/mL Mouse Anti-JAM-A Clone: J10.4 (Cat. No. SAB4200468). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting Lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-JAM-A Clone: J10.4 (Cat. No. SAB4200468). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. A549 2. A431 3. HepG2
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | J10.4, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 37 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1.0-2.0 μg/mL using HepG2 or A431 or A549 total cell extracts |
| UniProt accession no. | Q9Y624  |
| Application: | Anti-JAM-A antibody, Mouse monoclonal has been used in: • western blotting • staining for confocal microscopy • immunoprecipitation • immunocytochemistry • flow cytometry |
| Biochem/physiol Actions: | Anti-JAM-A antibody, Mouse monoclonal recognizes human JAM-A. |
| Biochem/physiol Actions: | Junctional adhesion molecule A(JAM-A) function has been linked with regulation of cell migration/invasion, platelet adhesion, cell polarity, cell proliferation, and epithelial or endothelial barrier function. However, the mechanisms by which JAM-A mediates these processes have not been well elucidated. JAM-A functional significance is connected with inflammation, angiogenesis, hypertension, ischemia/reperfusion, and atherosclerosis. JAM-A has also been linked to breast, endometrial, renal cell carcinomas and gastric cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-JAM-A antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM. Junctional adhesion molecule A (JAM-A) is expressed at tight junctions of endothelial and epithelial cells as well as on a variety of hematopoietic cells. It belongs to the transmembrane IgG glycoprotein family and is also expressed in monocytes, lymphocytes, neutrophils, dendritic cells, platelets, and spermatozoa. The JAM-A or F11R gene is mapped to human chromosome 1q23.3. |
| Immunogen: | derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM fusion protein. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |