Anti-EpCAM ICD antibody, Mouse monoclonal
SIGMA/SAB4200473 - clone 4A7, purified from hybridoma cell culture
Synonym: Monoclonal Anti-DIAR5; Monoclonal Anti-EGP-2; Monoclonal Anti-EGP314; Monoclonal Anti-EGP40; Monoclonal Anti-ESA; Monoclonal Anti-EpCAM ICD antibody produced in mouse; Monoclonal Anti-HNPCC8; Monoclonal Anti-KS1/4; Monoclonal Anti-KSA; Monoclonal Anti-M4S1; Monoclonal Anti-MIC18; Monoclonal Anti-MK-1; Monoclonal Anti-TACSTD1; Monoclonal Anti-TROP1; Monoclonal Anti-epithelial cell adhesion molecule
Product Type: Chemical
Immunofluorescence MCF7 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg Monoclonal Anti-EPCAM-ICD Clone: 4A7 (Cat. No. SAB4200473). The antibody was developed using Goat Anti-Mouse IgG, Atto488 conjugate (Cat. No. 62197). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-EPCAM-ICD Clone: 4A7 (Cat. No. SAB4200473). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. A431 2. HCT-116 3. SW480 4. HT-29 5. MCF7 6. 4T1
Immunoprecipitation Monoclonal Anti-EPCAM-ICD Clone: 4A7 (Cat. No. SAB4200473) was used to immunoprecipitate EPCAM-ICD from A431 whole cell extract. Detection antibody: Monoclonal Anti-EPCAM-ICD Clone: 4A7 (Cat. No. SAB4200473). Lanes 1. 5 μg antibody 2. 2.5 μg antibody 3. Negative control: 5 μg non-relevant antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4A7, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~40 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells. |
| western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells. | |
| UniProt accession no. | P16422  |
| Application: | Monoclonal Anti-EpCAM ICD antibody has been used in • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Epithelial cell adhesion molecule′s (EpCAM) intracellular domain (ICD) fragment translocates to the nucleus after cleavage from EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- EpCAM ICD (mouse IgG1 isotype) is derived from the hybridoma 4A7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. Epithelial cell adhesion molecule (EpCAM) is an epithelial transmembrane glycoprotein, composed of an ectodomain, a transmembrane domain and a cytoplasmic domain. EpCAM is frequently expressed in human malignancies, normal stem and progenitor cells, and at lower levels, in normal epithelia. |
| Immunogen: | synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |