Anti-ALIX antibody produced in rabbit
SIGMA/SAB4200476 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-AIP1; Anti-ALG-2 interacting protein 1; Anti-DRIP4; Anti-HP95; Anti-PDCD6IP; Anti-
Product Type: Chemical
Immunoblotting Whole extract of mouse A20 cells was separated on SDS-PAGE and probed with Rabbit Anti-ALIX (Cat. No. SAB4200476). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 5 μg/mL antibody 4. 5 μg/mL antibody + ALIX immunizing peptide
Immunoprecipitation Rabbit Anti-ALIX (Cat. No. SAB4200476) was used to immunoprecipitate ALIX from Jurkat whole cell extract. Detection antibody: Rabbit Anti-ALIX (C-terminal) (Cat. No. SAB4200477). Lanes 1. 5 μg antibody 2. 10 μg antibody 3. Negative control: 10 μg non-relevant antibody 4. Positive control: Jurkat whole cell extract
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~95 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of human Jurkat cells. |
| western blot: 2.5-5 μg/mL using whole extracts of mouse A20 cells. | |
| UniProt accession no. | Q8WUM4  |
| Application: | Anti-ALIX antibody produced in rabbit has been used in immunoprecipitation and immunoblotting. |
| Biochem/physiol Actions: | ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X binds to endophilins, proteins that regulate membrane shape during endocytosis. Over-expression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. |
| Biochem/physiol Actions: | PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region. |
| Immunogen: | peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in monkey, bovine and dog and differs by 2 amino acids in mouse and rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |