Anti-ALIX (C-terminal) antibody produced in rabbit
SIGMA/SAB4200477 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-PDCD6IP, programmed cell death 6 interacting protein, AIP1, DRIP4, HP95, ALG-2 interacting protein 1; Anti-
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol/acetone. Fixed cells were stained with 2 μg Rabbit Anti-ALIX (C-terminal) (Cat. No. SAB4200477). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of mouse A20 cells was separated on SDS-PAGE and probed with Rabbit Anti-ALIX (C-terminal) (Cat. No. SAB4200476). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + ALIX (C-terminal) immunizing peptide
Immunoprecipitation Rabbit Anti-ALIX (C-terminal) (Cat. No. SAB4200477) was used to immunoprecipitate ALIX from Jurkat whole cell extract. Detection antibody: Monoclonal Anti-ALIX. Lanes 1. 1 μg antibody 2. 2 μg antibody 3. Negative control: 2 μg non-relevant antibody 4. Positive control: Jurkat whole cell extract
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~95 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1-2 μg using lysates of human Jurkat cells |
| indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells | |
| western blot: 1-2 μg/mL using whole extracts of mouse A20 cells | |
| UniProt accession no. | Q8WUM4  |
| Application: | Anti-ALIX (C-terminal) antibody produced in rabbit may be used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Anti-ALIX (C-terminal) recognizes human and mouse ALIX. |
| Biochem/physiol Actions: | Apoptosis-linked gene 2-interacting protein X (ALIX) protein binds to endophilin proteins that regulate membrane shape during endocytosis. Over-expression of the ALIX gene and endophilin leads to cytoplasmic vacuolization, which may be a reason for protection against cell death. |
| Biochem/physiol Actions: | PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region. |
| Immunogen: | peptide corresponding to a sequence at the C-terminal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in mouse, pig, monkey, bovine and dog and differs by a single amino acid in rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |