Anti-SATB1 antibody, Mouse monoclonal
SIGMA/SAB4200481 - clone SAT-5, purified from hybridoma cell culture
Synonym: Anti-SATB homeobox 1; Anti-Special AT-rich sequence-binding protein 1; Monoclonal Anti-SATB1 antibody produced in mouse
Product Type: Chemical
Immunoblotting Jurkat cell extract was separated on SDS-PAGE and probed with Mouse Anti-SATB1 Clone: SAT-5 (Cat. No. SAB4200481). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + SATB1 immunizing peptide (human, 8-25) 3. Negative control
Flow Cytometry 1×106 THP-1 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton™ X-100. Fixed cells were labeled with 5 μg Mouse Anti-SATB1 Clone: SAT-5 (Cat. No. SAB4200481) followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody; Purple histogram: antibody specific staining.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | SAT-5, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, monkey, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5-10 μg using THP-1 cells |
| immunocytochemistry: suitable | |
| western blot: 2.0-4.0 μg/mL using Jurkat total cell extracts | |
| UniProt accession no. | Q01826  |
| Application: | Monoclonal Anti-SATB1 antibody produced in mouse has been used in: • immunoblotting • immunocytochemistry • flow cytometry |
| Biochem/physiol Actions: | Special AT-rich sequence-binding protein 1 (SATB1) is a tumor promotor gene that plays an important role in regulating gene expression. The SATB1 known to be involved in organizing higher-order chromatin structure including the subnuclear organization of individual genes within multigene clusters in a tissue specific manner, locally and at a distance. It controls the global chromatin architecture via promyelocytic leukemia protein (PML). SATB1 also regulates the periodic anchoring of matrix attachment regions (MARs) in DNA to the nuclear matrix. This organizes chromatin into distinct loops. Furthermore, SATB1 recruits co-repressor complexes to its binding sites. SATB1 is abnormally expressed in human metastatic breast cancer and also promotes breast cancer tumor growth and metastasis. It is involved in the tumorigenesis of colorectal, coetaneous malignant melanoma and lung cancers. SATB1 also serves as a possible prognostic marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-SATB1 (mouse IgG2a isotype) is derived from the hybridoma SAT-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminus of human special AT-rich sequence-binding protein 1 (SATB1), conjugated to keyhole limpet hemocyanin (KLH). SATB1 encodes as nuclear matrix protein that interacts with scaffold-associating DNA. SATB1 gene is mapped to human chromosome 3p24.3. |
| Immunogen: | a synthetic peptide corresponding to a sequence at the N-terminus of human SATB1, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |