Anti-JAK2 antibody, Mouse monoclonal
SIGMA/SAB4200483 - clone 10.1.5, purified from hybridoma cell culture
Synonym: Anti-JAK-2; Anti-JTK10; Anti-Janus kinase 2; Monoclonal Anti-JAK2 antibody produced in mouse
Product Type: Chemical
Immunofluorescence MCF7 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton™ X-100. Fixed cells were stained with 5 μg/mL Mouse Anti-JAK2 Clone: 10.1.5 (Cat. No. SAB4200483). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Extract of TF1 cells was separated on SDS-PAGE and probed with Mouse Anti-JAK2 Clone: 10.1.5 (Cat. No. SAB4200483). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + 20 μg/mL JAK2 immunizing peptide (561-575)
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 10.1.5, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~125 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using MCF7 cells. |
| western blot: 2.0-4.0 μg/mL using TF1 total cell extracts. | |
| UniProt accession no. | O60674  |
| Application: | Monoclonal Anti-JAK2 antibody produced in mouse has been used in: • immunoblotting • immunocytochemistry • flow cytometry |
| Biochem/physiol Actions: | Janus kinases engage in interactions with the intracellular tails of several cytokine receptors, which result in the activation of members of the signal transducer and activator of transcription (STAT) family of transcription factors. The latter bind specific cognate sequences at the promoter region of genes that regulate proliferation and differentiation. JAK activation plays an important role in cell proliferation, differentiation, migration and apoptosis. A member of this family, JAK2, has been found to induce signaling regulating gene expression of various pro-survival and anti-apoptotic molecules. Furthermore, inhibition of JAK2 signaling triggered cell death in human cancer cells. These findings indicate JAK2 inhibition as a potential therapeutic approach for various cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-JAK2 (mouse IgG1 isotype) is derived from the hybridoma 10.1.5 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. The Janus kinases (Jak) family of proteins includes four members: Jak1, Jak2, Jak3 and the tyrosine kinase 2 (Tyk2). Jak2 is ubiquitously expressed. It is also expressed in normal and neoplastic mammary epithelial cells. |
| Immunogen: | synthetic peptide corresponding to an internal sequence of human JAK2 (GeneID 3717), conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |