Anti-acetyl-Histone H3 (Ac-Lys27) antibody produced in rabbit
SIGMA/SAB4200485 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-H3.4; Anti-H3/g; Anti-H3FT; Anti-H3K27ac; Anti-H3t; Anti-HIST3H3; Anti-Hist1h2ai; Anti-Hist1h2ail; Anti-Hist1h3a; Anti-Hist1h3g; Anti-Hist1h3h; Anti-Hist1h3i
Product Type: Chemical
Immunohistochemistry Localization of acetyl-Histone H3 (Ac-Lys27) in human breast carcinoma. Fixed tissue was stained with 10 μg/mL Anti-Acetyl-Histone H3 (Ac-Lys27) (Cat. No. SAB4200485) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunofluorescence Nuclear localization of acetyl-Histone H3 (Ac-Lys27) in HeLa cells. Fixed cells were stained with 2 μg/mL Anti-Acetyl-Histone H3 (Ac-Lys27) (Cat. No. SAB4200485) followed by Goat Anti-Rabbit IgG, FITC conjugate (×40 magnification). Actin staining with phalloidin rhodamine conjugate.
Immunoblotting Lysates of NIH3T3 cells (lane 1) and NIH3T3-treated with 5mM Na+-butyrate for 24h., (lane 2-4) were separated on SDS-PAGE, blotted with 1 μg/mL Anti-Acetyl-Histone H3 (Ac-Lys27), (Cat. No. SAB4200485) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. NIH3T3 2. NIH3T3 (Na+-butyrate) 3. NIH3T3 (Na+-butyrate). Antibody + non-acetylated Histone H3 peptide 4. NIH3T3 (Na+-butyrate). Antibody + immunizing acetylated Histone H3 (Ac-Lys27) peptide
ELISA ELISA plates were coated with peptides at 0.5 μg/mL, incubated with different concentrations of Anti-Acetyl-Histone H3 (Ac-Lys27) (Cat. No. SAB4200485) and developed using Goat Anti-Rabbit IgG, ALP conjugate, and a colorimetric substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~15 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | acetylation (Lys27) |
| technique(s) | immunohistochemistry: 10-20 μg/mL |
| indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells | |
| western blot: 1-2 μg/mL using extracts of NIH3T3 cells | |
| UniProt accession no. | Q16695  |
| Application: | Anti-acetyl-Histone H3 (Ac-Lys27) antibody produced in rabbit may be used in: • immunoblotting • immunofluorescence • immunohistochemistry |
| Biochem/physiol Actions: | Anti-acetyl-Histone H3 (Ac-Lys27) specifically recognizes human and mouse (Ac-Lys27) Histone H3 (H3K27Ac). |
| Biochem/physiol Actions: | Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination. Active chromatin is highly correlated with the hyperacetylation of histone H3 at the N-terminal tail. Histone H3 can be reversibly acetylated at Lys residues 9, 14, 18, 23, 27, 36 and 56 by a family of enzymes known as histone acetyltransferases (HATs). Histone H3 can be modified at Lys27 either by methylation or acetylation. Histones are thought to play an important role in development and cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The HIST3H3 gene is mapped on the human chromosome at 1q42.13. Histones are proteins that form the nucleosome along with DNA. A nucleosome consists of core histone octamers made of two heterodimers of H2A and H2B along with two heterodimers of H3 and H4. This octamer is bound by the DNA and H1 protein. |
| Immunogen: | synthetic peptide containing acetylated Lys27 of human histone H3, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H3 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |