Anti-IAPP antibody produced in rabbit
SIGMA/SAB4200493 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-Amylin; Anti-DAP; Anti-
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Human Pancreas sections stained with 5 μg/mL Rabbit Anti-IAPP (Cat. No. SAB4200493) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 5 μg/mL Rabbit Anti-IAPP (Cat. No. SAB4200494) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-IAPP antibodies, SAB4200494 (A) and SAB4200493 (B) target different regions of IAPP show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Localization of islet amyloid polypeptide (IAPP) in human pancreas. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (Cat. No. SAB4200493) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunohistochemistry Localization of islet amyloid polypeptide (IAPP) in human colon. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (Cat. No. SAB4200493) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunohistochemistry Inhibition of islet amyloid polypeptide (IAPP) staining in human pancreas. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (Cat. No. SAB4200493) pre-incubated with IAPP immunizing peptide, followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunofluorescence RIN-5F rat insulinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Anti-IAPP antibody produced in Rabbit (Cat. No. SAB4200493). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent ( Antibodies) Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin-embedded human pancreas. |
| indirect immunofluorescence: 1-2 μg/mL using rat insulinoma RIN-5F cells. | |
| UniProt accession no. | P10997  |
| Application: | Anti-IAPP antibody produced in rabbit has been used in immunohistochemistry and immunofluorescence. |
| Biochem/physiol Actions: | IAPP/Amylin plays a role in glycemic regulation by slowing gastric emptying and promoting satiety, thereby preventing post-prandial spikes in blood glucose levels. IAPP has been linked to type 2 diabetes (T2DM) and the loss of islet b-cells. The human IAPP (20-29) region that has been found to be essential to amyloid formation is thought to be a key factor in the initiation of amyloid aggregation. Islet amyloid formation, initiated by the aggregation of IAPP, may contribute to this progressive loss of islet β-cells. Amyloid deposits deriving from IAPP are commonly found in pancreatic islets of patients suffering of T2DM, or containing an insulinoma cancer. IAPP, like β-amyloid peptide associated with Alzheimer′s disease, can induce apoptosis in insulin producing β-cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Islet amyloid polypeptide (IAPP, amylin), is a 37-residue peptide hormone co-secreted with insulin from the pancreatic β-cells. IAPP is processed from an 89-amino acid precursor pro-islet amyloid polypeptide (proIAPP) in pancreatic β-cells that undergoes complex posttranslational modifications including protease cleavage, C-terminal amidation and formation of an intramolecular disulfide bridge to produce the mature IAPP. |
| Immunogen: | synthetic peptide corresponding to a sequence at the C-terminus of rat pro-IAPP, conjugated to KLH. The corresponding sequence is identical in mouse IAPP and has 74% sequence identity with human IAPP. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |