Anti-IAPP (N-terminal) antibody produced in rabbit
SIGMA/SAB4200494 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-Amylin; Anti-DAP; Anti-
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Human Pancreas sections stained with 5 μg/mL Rabbit Anti-IAPP (Cat. No. SAB4200493) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 5 μg/mL Rabbit Anti-IAPP (Cat. No. SAB4200494) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-IAPP antibodies, SAB4200494 (A) and SAB4200493 (B) target different regions of IAPP show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Localization of islet amyloid polypeptide (IAPP) in human pancreas. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (N-terminal) (Cat. No. SAB4200494) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunohistochemistry Localization of islet amyloid polypeptide (IAPP) in human stomach (antrum). Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (N-terminal) (Cat. No. SAB4200494) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunohistochemistry Localization of islet amyloid polypeptide (IAPP) in human stomach (fundus). Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (N-terminal) (Cat. No. SAB4200494) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunohistochemistry Localization of islet amyloid polypeptide (IAPP) in human jejunum. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (N-terminal) (Cat. No. SAB4200494) Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunohistochemistry Inhibition of islet amyloid polypeptide (IAPP) staining in human pancreas. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (N-terminal) (Cat. No. SAB4200494) pre-incubated with IAPP immunizing peptide, followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
Immunohistochemistry Inhibition of islet amyloid polypeptide (IAPP) staining in human jejunum. Formalin-fixed paraffin-embedded tissue was stained with 5 μg/mL Anti-IAPP (N-terminal) (Cat. No. SAB4200494) pre-incubated with IAPP immunizing peptide, followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®HRP (×20 magnification).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent ( Antibodies) Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 5-10 μg/mL using formalin-fixed paraffin-embedded human pancreas. |
| UniProt accession no. | P10997  |
| Application: | Anti-IAPP (N-terminal) antibody produced in rabbit may be used in immunohistochemistry. |
| Biochem/physiol Actions: | Anti-IAPP(N-terminal) specifically recognizes human IAPP. |
| Biochem/physiol Actions: | Islet amyloid polypeptide (IAPP)/Amylin plays a role in glycemic regulation by slowing gastric emptying and promoting satiety, thereby preventing post-prandial spikes in blood glucose levels. The human IAPP (20-29) region that has been found to be essential to amyloid formation is thought to be a key factor in the initiation of amyloid aggregation. Amyloid deposits deriving from IAPP are commonly found in pancreatic islets of patients suffering of T2DM, or containing an insulinoma cancer. IAPP, like β-amyloid peptide associated with Alzheimer′s disease, can induce apoptosis in insulin producing β-cells, an effect that is relevant to the development of type 2 diabetes. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Islet amyloid polypeptide (IAPP), also known as amylin, is a 37 amino acid peptide hormone co-secreted with insulin from the pancreatic Β-cells. IAPP is processed from an 89-amino acid precursor pro-islet amyloid polypeptide (proIAPP) in pancreatic Β-cells that undergoes complex posttranslational modifications including protease cleavage, C-terminal amidation and formation of an intramolecular disulfide bridge to produce the mature IAPP. |
| General description: | The IAPP gene is mapped on the human chromosome at 12p12.1. |
| Immunogen: | synthetic peptide corresponding to a sequence at the N-terminus of human pro-IAPP (GeneID 3375), conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |