Anti-THY1 antibody, Mouse monoclonal
SIGMA/SAB4200497 - clone TH350, purified from hybridoma cell culture
Synonym: Anti-CD90; Anti-CDw90; Anti-FLJ33325; Anti-Thy-1 T-cell antigen; Anti-Thy-1 antigen; Anti-Thy-1 cell surface antigen; Anti-thy-1 membrane glycoprotein; Monoclonal Anti-THY1 antibody produced in mouse
Product Type: Chemical
Immunoblotting Whole extract of NT2 cells was separated on SDS-PAGE and probed with Mouse Anti-THY1 Clone: TH350 (Cat. No. SAB4200497). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + 90 μg/mL THY1 immunizing peptide (human, 46-59) 3. 2 μg/mL antibody + 90 μg/mL THY1 immunizing peptide (human, 88-103)
Immunoblotting Lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-THY1 Clone: THY350 (Cat. No. SAB4200497). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. NT2 2. SH-SY5Y 3. A549 4. Mouse total brain lysate
Flow Cytometry 1×106 A549 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton™ X-100. Fixed cells were labeled with Mouse Anti-THY1 Clone: TH350 (Cat. No. SAB4200497) 20 μgr/mL followed by Rabbit Anti-Mouse IgG, FITC conjugate. Green histogram: negative control without first antibody; Purple histogram: antibody specific staining.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TH350, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~27 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| flow cytometry: 20-40 μg/test using A549 cells. | |
| immunohistochemistry: suitable | |
| western blot: 2.0-4.0 μg/mL using NT2 total cell extracts. | |
| UniProt accession no. | P04216  |
| Application: | Monoclonal Anti-THY1 antibody produced in mouse may be used in: • immunoblotting • flow cytometry • immunofluorescence |
| Biochem/physiol Actions: | Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1. |
| Biochem/physiol Actions: | Thy-1 cell surface antigen (THY1) has a role in the activation of T-cells, release of neurotransmitters at synapses, enhancement of adhesion of astrocytes, wound healing, fibrogenesis and tumor suppression. It also plays an important role in cell migration and cytoskeletal reorganization. |
| Biochem/physiol Actions: | THY-1 is also involved in the inhibition of neurite outgrowth, apoptotic signaling, leukocyte and melanoma cell adhesion and migration and fibroblast proliferation and migration. This protein plays a role as a mediator of cell-cell and cell-matrix interactions. Thy-1 is a cell surface marker in identifying local and circulating liver cancer stem cells. This protein′s expression can be regulated by the epigenetic mechanism of promoter region methylation, in which down-regulation of Thy-1 is associated with a more invasive/metastatic clinical phenotype. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-THY1 (mouse IgG1 isotype) is derived from the hybridoma TH350 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to keyhole limpet hemocyanin (KLH). Monoclonal Anti-THY1 recognizes human, rat and mouse Thy-1. Thy-1 cell surface antigen (THY1) is a glycoprotein that is expressed on the membranes of adult cells, mesenchymal stromal cells and cancer cells. It has a molecular weight of about 25-37kDa. The gene encoding THY1 is localized on human chromosome 11q23.3. |
| General description: | THY-1 is expressed in various cell types, including human fibroblasts, neurons, blood stem cells and endothelial cells as well as murine T cells. |
| Immunogen: | synthetic peptide corresponding to a sequence at the N-terminus of human THY1, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For extended storage, freeze at -20 0C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |