Anti-Collagen IV antibody, Mouse monoclonal
SIGMA/SAB4200500 - clone J3-2, purified from hybridoma cell culture
Synonym: Anti-COL4; Monoclonal Anti-Collagen IV antibody produced in mouse
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded sections of Human liver with 3 μg/mL Mouse Anti-Collagen IV Clone: J3-2 (Cat. No. SAB4200500), using biotin/ExtrAvidin®-Peroxidase (Cat. No. B0529).
Immunofluorescence MDBK cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 5 μg/mL Mouse Anti-Collagen IV Clone: J3-2 (Cat. No. SAB4200500). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Extract of Human Placenta was separated on SDS-PAGE and probed with Mouse Anti-Collagen IV Clone: J3-2 (Cat. No. SAB4200500). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. Negative control
Immunoblotting Cell extracts were separated on SDS-PAGE and probed with 1 μg/mL Mouse Anti-Collagen IV Clone: J3-2 (Cat. No. SAB4200500). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 293T 2. 3T3-L1 3. NRK 4. JURKAT 5. MDBK 6. MDCK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | J3-2, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, dog, bovine, human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 2.0-4.0 μg/mL using human liver tissue sections. |
| western blot: 1.0-2.0 μg/mL using human placenta extracts. |
| Application: | Monoclonal Anti-Collagen IV antibody produced in mouse has been used: • in the immunohistochemical staining • in the immunostaining • in the immunofluorescence detection • as a secondary antibody in the western blot analysis |
| Biochem/physiol Actions: | Collagen IV plays a key role in basement membrane assembly. The six chains assemble as three different protomers namely, the α 1.α1.α2(IV), α3.α4.α5(IV) and α 5.α5.α6(IV). The trimers are formed in different combinations of the α-chains. High expression levels of α3, α4 and α5 is observed in specialized glomerular BM (GBM). Mutations in the COlA5 gene is associated with Alport syndrome and α3(IV) chain is associated pathogenesis of Goodpasture syndrome, an autoimmune disease. Mutation in the COlA5 is also implicated in Diffuse Esophageal Lewmyomatosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human collagen IV comprises six genes. The COL4A1-COL4A2 is mapped to chromosome 13. The COL4A3-COL4A4 and COL4A5-COL4A6 are localized on chromosome 2 and chromosome X, respectively. It belongs to the collagen superfamily and is associated with the basement membranes (BMs). The six α-chains namely α1(IV) to α6(IV) comprises an N-terminal cysteine and lysine domain, a typical Gly-X-Y triple repeats and a C-terminal noncollagenous (NC1) domain. |
| Immunogen: | placenta preparation rich in basement membrane collagen. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |