Anti-PSPC1 antibody, Mouse monoclonal
SIGMA/SAB4200503 - clone 1L4, purified from hybridoma cell culture
Synonym: Monoclonal Anti-PSP1; Monoclonal Anti-PSPC1 antibody produced in mouse; Monoclonal Anti-paraspeckle component 1
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluorescence. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-PSPC1 antibody, Mouse monoclonal, Clone: 1L4 (Cat. No. SAB4200503) (A). The antibody developed using Goat Anti-Mouse IgG, Cy3 Conjugate antibody, and 2.5 μg/mL Anti-PSPC1 (N-terminal) antibody produced in Rabbit (Cat. No. SAB4200068) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody. Results Two Anti-PSPC1 antibodies, SAB4200503 (A) and SAB4200068 (B), target different regions of PSPC1 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 5 μg Monoclonal Anti-PSPC1 Clone: 1L4 (Cat. No. SAB4200503). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate.
Immunoblotting Whole extract of mouse Hepa1-6 cells was separated on SDS-PAGE and probed with Monoclonal Anti-PSPC1 Clone: 1L4 (Cat. No. SAB4200503). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 2 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1L4, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~60 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using Human HeLa cells. |
| western blot: 0.5-1 μg/mL using Whole extracts of mouse Hepa1-6 cells. | |
| UniProt accession no. | Q8WXF1  |
| Application: | Monoclonal Anti-PSPC1 antibody produced in mouse has been used in immunohistochemistry, immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Drosophila behavior, human splicing (DBHS) proteins bind both double- and single-stranded DNA and RNA, and are involved in various aspects of RNA and DNA metabolism, such as transcription, pre-mRNA 3′ processing, transcription termination and mRNA splicing. DBHS proteins regulate circadian rhythm and carcinogenesis. PSPC1 activates androgen receptor mediated transcription. PSPC1 forms a heterodimer with NONO that localizes to paraspeckles in a RNA polymerase II-dependent manner. |
| Biochem/physiol Actions: | Since paraspeckle component 1 (PSPC1) has lower background nucleoplasmic signal in various cell types compared to other members of DBHS (Drosophila Behavior Human Splicing) family of proteins, it is frequently used as a marker for paraspeckles. The encoded protein plays a vital role in regulation of transcription. Deletion of the gene is associated with the hindered psychomotor development, muscle hypotonia, unilateral microphthalmia with ptosis, congenital eye malformation and facial dysmorphic features. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PSPC1 (mouse IgG1 isotype) is derived from the hybridoma 1L4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of mouse PSPC1. DBHS (Drosophila behavior, human splicing) protein family, consisting also of NONO (nonPOU-domain-containing |
| General description: | Paraspeckle component 1 (PSPC1) is encoded by the gene mapped to human chromosome 13q12.11. The encoded protein is a member of DBHS (Drosophila Behavior Human Splicing) family of proteins. |
| Immunogen: | Synthetic peptide corresponding to a sequence at the C-terminal region of mouse PSPC1, conjugated to KLH. 1 The corresponding sequence is identical in rat and human PSPC1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |